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在人体细胞中使用自定位CRISPR-Cas进行持续的基因记录

Samuel D Perli¹, Cheryl H Cui², Timothy K Lu³

¹Synthetic Biology Group, MIT Synthetic Biology Center, Massachusetts Institute of Technology (MIT), Cambridge, MA 02139, USA. Research Laboratory of Electronics, MIT, Cambridge, MA 02139, USA. Department of Electrical Engineering and Computer Science, MIT, Cambridge, MA 02139, USA.

Science (New York, N.Y.)

|August 20, 2016

概括

科学家开发了一种基于DNA的模拟记忆装置, 这种名为mSCRIBE的工具可以在体内监测细胞行为和信号动态.

科学领域:

分子生物学
合成生物学
生物技术

背景情况:

在体内监测分子事件对于理解细胞信号和行为至关重要.
现有的方法缺乏细胞内的纵向记录能力.

研究的目的:

开发一种独立的模拟记忆装置,用于将分子刺激记录为DNA突变.
创建一个用于纵向监测细胞行为和信号动态的工具.

主要方法:

一种使用自向导RNA (stgRNA) 和Streptococcus pyogenes Cas9核酶的新型装置被设计出来.
该系统能够基于stgRNA表达的局部,连续的DNA突变.
在人类细胞中,可编程和多重存储的记忆被诱导剂或炎症触发.

主要成果:

哺乳动物合成细胞记录器整合生物事件 (mSCRIBE) 已成功开发和验证.
在人体细胞中证明了 in vitro 和 in vivo 的功能.
展示了可编程和多重存储数据的能力.

结论:

mSCRIBE为研究细胞生物学提供了一种新的策略.
这种装置可用于生物记录的目标DNA序列的持续演变.
这项技术推动了体内细胞监测和合成生物学领域的发展.

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