相关实验视频

Updated: Mar 16, 2026

Sedimentation Equilibrium of a Small Oligomer-forming Membrane Protein: Effect of Histidine Protonation on Pentameric Stability

Sedimentation Equilibrium of a Small Oligomer-forming Membrane Protein: Effect of Histidine Protonation on Pentameric Stability

Published on: April 2, 2015

外围膜蛋白的水化动力学

Olivier Fisette¹, Christopher Päslack^1,2, Ryan Barnes³

¹Center for Theoretical Chemistry, Faculty of Chemistry and Biochemistry, Ruhr-University , 44780 Bochum, Germany.

Journal of the American Chemical Society

|August 23, 2016

概括

膜附近的水运动被蛋白质减缓,延伸到膜表面之外. 这种由蛋白质引起的作用比脂质相互作用弱,但对于理解与膜相关的生物过程具有重要意义.

科学领域:

生物物理
物理化学
结构生物学

背景情况:

周围膜蛋白在细胞功能中起着至关重要的作用.
了解膜界面上的水动力学是阐明生物过程的关键.

研究的目的:

在脂双层附近的附B12的水化中研究水动力学.
为了区分蛋白质诱导的水阻滞与脂质诱导的影响.

主要方法:

分子动力学 (MD) 模拟.
用Overhauser动态核极化 (DNP) 增强的核磁共振 (NMR) 光谱.

主要成果:

脂双层附近的水运动减速被膜结合的附录素B12延长到~10 Å.
蛋白质诱导的水阻滞比脂质头组相互作用弱,但在膜的10 Å之外占主导地位.
对水网络振动的分析揭示了从膜表面到散装水中的度梯度.

结论:

一个简单的添加模型解释了膜和蛋白质贡献的水扩散障碍.
膜附近的延迟水动力学影响分子识别,结合和蛋白质与蛋白质的相互作用.
确定了影响膜接口水的梯度.

更多相关视频

Nitrogen Cavitation and Differential Centrifugation Allows for Monitoring the Distribution of Peripheral Membrane Proteins in Cultured Cells

Nitrogen Cavitation and Differential Centrifugation Allows for Monitoring the Distribution of Peripheral Membrane Proteins in Cultured Cells

Published on: August 18, 2017

Method to Visualize and Analyze Membrane Interacting Proteins by Transmission Electron Microscopy

Method to Visualize and Analyze Membrane Interacting Proteins by Transmission Electron Microscopy

Published on: March 5, 2017

相关概念视频

Membrane Fluidity

Membrane Fluidity

Cell membranes are composed of phospholipids, proteins, and carbohydrates loosely attached to one another through chemical interactions. Molecules are generally able to move about in the plane of the membrane, giving the membrane its flexible nature called fluidity. Two other features of the membrane contribute to membrane fluidity: the chemical structure of the phospholipids and the presence of cholesterol in the membrane.

Membrane Fluidity

Membrane Fluidity

Membrane fluidity is explained by the fluid mosaic model of the cell membrane, which describes the plasma membrane structure as a mosaic of components—including phospholipids, cholesterol, proteins, and carbohydrates—that gives the membrane a fluid character.
Mosaic nature of the membrane
The mosaic characteristic of the membrane helps the plasma membrane remain fluid. The integral proteins and lipids exist as separate but loosely-attached molecules in the membrane. The membrane is...

Fluid Mosaic Model

Fluid Mosaic Model

Scientists identified the plasma membrane in the 1890s and its principal chemical components (lipids and proteins) by 1915. The model for plasma membrane structure, proposed in 1935 by Hugh Davson and James Danielli, was the first model to be widely accepted in the scientific community. The model was based on the plasma membrane's "railroad track" appearance in early electron micrographs. Davson and Danielli theorized that the plasma membrane's structure resembled a sandwich...

Protein Diffusion in the Membrane

Protein Diffusion in the Membrane

Proteins show rotational as well as lateral diffusion across the membrane. The lateral diffusion of proteins was confirmed through the cell fusion experiment where mouse and human cells were fused, resulting in hybrid cells. When the human and mouse cells fused, the specific membrane proteins on human and mouse cells were marked with the red and green-fluorescent markers, respectively. Initially, the red and green fluorescence was located on the respective hemisphere of the cell. As time...

Introduction to Membrane Proteins

Introduction to Membrane Proteins

The cell membrane, or plasma membrane, is an ever-changing landscape. It is described as a fluid mosaic where various macromolecules are embedded in the phospholipid bilayer. Among the macromolecules are proteins. The protein content varies across cell types. For example, mitochondrial inner membranes contain ~76% protein content, while myelin contains ~18% protein content. Individual cells contain many types of membrane proteins—red blood cells contain over 50—and different cell...

Aquaporins

Aquaporins

Aquaporins or AQPs are a family of integral membrane proteins whose primary function is to transport water, while some called aquaglyceroporins also transport glycerol. In addition, aquaporins have also been suspected to be involved in transporting volatile substances, such as carbon dioxide and ammonia, across membranes. Such AQPs that act as gas channels are often highly expressed in cells involved in the gaseous exchange, such as red blood cells, epithelial cells, and pulmonary capillaries.