细胞透的激活与离子对-π 相互作用和
Nicolas Chuard1, Kaori Fujisawa1, Paola Morelli1
1Department of Organic Chemistry, University of Geneva , CH-1211 Geneva, Switzerland.
Journal of the American Chemical Society
|August 30, 2016
概括
两种新概念激活细胞透 (CPPs),以增强细胞膜传输. 平行离子对π相互作用和排斥驱动的离子配对与自组合的性物是关键策略.
科学领域:
- 生物化学
- 分子生物学
- 细胞生物学
背景情况:
- 细胞透 (CPP) 促进分子穿越细胞膜.
- 现有的模型表明,反射驱动的离子配对与离子脂质对于CPP转位至关重要.
- 像甲酸这样的疏水性离子可以通过防止内体捕获来加速CPP的吸收.
研究的目的:
- 探索用于激活富含氨酸的CPP的新概念.
- 研究对π相互作用和自组合在CPP激活中的作用.
- 确定新的策略来提高蜂交付中的CPP效率.
主要方法:
- 设计用于离子对π相互作用的离子两性分子的合成.
- 使用模型膜和活细胞 (HeLa) 测试CPP激活.
- 评估化脂肪酸的自我组装和CPP激活特性.
主要成果:
- 平行,但不是反平行,离子对π相互作用有效地激活了模型膜和细胞中的CPP.
- perfluorinated 脂肪酸在 HeLa 细胞中显著激活了 CPP,但在模型膜中没有.
- 这些发现支持平行离子对π相互作用和自组合性物对CPP激活的有效性.
结论:
- 平行离子对π相互作用代表了CPP激活的可行策略.
- 通过排斥驱动的离子配对与自组装的性纤维提供了激活CPP的另一种创新方法.
- 这些研究结果强烈支持以反为媒介的吸收作为富含氨酸的CPP的机制.
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