Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

The Tumor Microenvironment02:17

The Tumor Microenvironment

8.0K
Every normal cell or tissue is embedded in a complex local environment called stroma, consisting of different cell types, a basal membrane, and blood vessels. As normal cells mutate and develop into cancer cells, their local environment also changes to allow cancer progression. The tumor microenvironment (TME) consists of a complex cellular matrix of stromal cells and the developing tumor. The cross-talk between cancer cells and surrounding stromal cells is critical to disrupt normal tissue...
8.0K
The Tumor Microenvironment02:17

The Tumor Microenvironment

3.0K
3.0K
Tumor Immunotherapy01:27

Tumor Immunotherapy

2.1K
Immunotherapy is a treatment that boosts or manipulates the immune system to fight diseases, including cancer. For instance, by stimulating an immune response through vaccinations against viruses that cause cancers, like hepatitis B virus and human papillomavirus, these diseases can be prevented. Nonetheless, some cancer cells can avoid the immune system due to their rapid mutation and division. The immune response to many cancers involves three phases: elimination, equilibrium, and escape.
2.1K
Cytotoxic T Cells-mediated Immune Response01:27

Cytotoxic T Cells-mediated Immune Response

7.8K
Cytotoxic T cells are a vital component of the immune system. They have the remarkable ability to identify and target antigens on infected or abnormal cells. These antigens often originate from intracellular pathogens such as viruses or abnormal proteins cancer cells produce.
Immunological surveillance is the ability of immune cells to monitor and eliminate infected cells with intracellular pathogens, neoplastically transformed cells, and cells with non-self antigens. Cytotoxic T cells and NK...
7.8K
Cell-mediated Immune Responses01:40

Cell-mediated Immune Responses

85.7K
Overview
85.7K
Immune Surveillance by NK Cells and Phagocytes01:25

Immune Surveillance by NK Cells and Phagocytes

9.3K
Immune surveillance is an integral part of the innate immune system, involving the continuous monitoring of peripheral tissues to detect and respond to pathogens, infected cells, or cancerous cells. This surveillance is conducted primarily by natural killer (NK) cells and phagocytes, which employ distinct but complementary mechanisms to identify and eliminate threats.
Natural Killer Cells: The Fast Responders
NK cells are large granular lymphocytes found in the blood and lymphatic system. These...
9.3K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

A phase 1 trial of HPV16 E7 T-cell receptor-engineered T cells in patients with relapsed/refractory HPV16-positive cancers (KITE-439 trial).

Frontiers in oncology·2026
Same author

HLA micropolymorphisms confine neoantigen conformational adaptability and guide T cell receptor selectivity.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Author Correction: The efficacy of chemotherapy is limited by intratumoral senescent cells expressing PD-L2.

Nature cancer·2026
Same author

Cancer type-specific variation in patterns of driver alterations across 50,000 tumors.

Cancer cell·2026
Same author

Mitochondrial ATP production promotes T cell differentiation and function by regulating chromatin accessibility.

bioRxiv : the preprint server for biology·2026
Same author

What factors differentiate early from late pulmonary metastases in soft-tissue sarcoma of the limbs? : a comparative cohort analysis.

The bone & joint journal·2026

相关实验视频

Updated: Mar 15, 2026

Studying the Effects of Tumor-Secreted Paracrine Ligands on Macrophage Activation using Co-Culture with Permeable Membrane Supports
07:44

Studying the Effects of Tumor-Secreted Paracrine Ligands on Macrophage Activation using Co-Culture with Permeable Membrane Supports

Published on: November 28, 2019

8.2K

在瘤微环境中的离子免疫抑制限制了T细胞效应器功能.

Robert Eil1, Suman K Vodnala1, David Clever1

  • 1National Cancer Institute, National Institutes of Health (NIH), Bethesda, Maryland 20892, USA.

Nature
|September 15, 2016
PubMed
概括
此摘要是机器生成的。

瘤缩释放,抑制T细胞的功能. 过度表达T细胞中的Kv1.3通道增强了抗瘤免疫力,并改善了黑色素瘤模型中的存活率.

更多相关视频

Analysis of Human T Cell Activity in an Allogeneic Co-Culture Setting of Pre-Treated Tumor Cells
09:04

Analysis of Human T Cell Activity in an Allogeneic Co-Culture Setting of Pre-Treated Tumor Cells

Published on: March 7, 2025

1.8K
Evaluation of Tumor-infiltrating Leukocyte Subsets in a Subcutaneous Tumor Model
07:49

Evaluation of Tumor-infiltrating Leukocyte Subsets in a Subcutaneous Tumor Model

Published on: April 13, 2015

21.0K

相关实验视频

Last Updated: Mar 15, 2026

Studying the Effects of Tumor-Secreted Paracrine Ligands on Macrophage Activation using Co-Culture with Permeable Membrane Supports
07:44

Studying the Effects of Tumor-Secreted Paracrine Ligands on Macrophage Activation using Co-Culture with Permeable Membrane Supports

Published on: November 28, 2019

8.2K
Analysis of Human T Cell Activity in an Allogeneic Co-Culture Setting of Pre-Treated Tumor Cells
09:04

Analysis of Human T Cell Activity in an Allogeneic Co-Culture Setting of Pre-Treated Tumor Cells

Published on: March 7, 2025

1.8K
Evaluation of Tumor-infiltrating Leukocyte Subsets in a Subcutaneous Tumor Model
07:49

Evaluation of Tumor-infiltrating Leukocyte Subsets in a Subcutaneous Tumor Model

Published on: April 13, 2015

21.0K

科学领域:

  • 免疫学 免疫学 免疫学
  • 癌症生物学 癌症生物学
  • 细胞生理学 细胞生理学

背景情况:

  • 尽管T细胞透,瘤的进展仍在发生.
  • 瘤中的细胞亡与患者的生存率差相关.
  • 亡会释放细胞内成分,可能会影响瘤的微环境.

研究的目的:

  • 为了研究 necrosis 诱导的细胞外对 T 细胞功能的影响.
  • 阐明介导的T细胞抑制背后的分子机制.
  • 探索针对癌症免疫治疗的水平的治疗策略.

主要方法:

  • 在小鼠和人类瘤中分析细胞外度 ([K+]e).
  • 评估T细胞受体 (TCR) 驱动的Akt-mTOR酸化.
  • 在体外和体内T细胞效应器程序的功能测试.
  • 对T细胞进行遗传操纵,以过度表达通道Kv1.3.3.

主要成果:

  • 来自的细胞外 ([K+]e) 含量升高抑制T细胞效应器功能.
  • 高[K+]e通过酸酶PP2A损害了Akt-mTOR通过酸酶PP2A传递的信号.
  • 抑制需要增加细胞内 ([K+]i),独立于血膜电位 (Vm).
  • 在黑色素瘤模型中,Kv1.3的过度表达增强了T细胞功能和瘤清除.

结论:

  • 结核诱发的高胆固醇血症会产生一种"离子检查点",抑制抗瘤T细胞的反应.
  • 向流是增强癌症免疫疗法的新策略.
  • 调节T细胞水平可以改善瘤根除和患者存活率.