用冷电子显微镜解生物大分子
Rafael Fernandez-Leiro1, Sjors H W Scheres1
1MRC Laboratory of Molecular Biology, Cambridge Biomedical Campus, Cambridge CB2 0QH, UK.
Nature
|September 16, 2016
概括
电子显微镜 (cryo-EM) 提供了对生物大分子结构的原子层次洞察力. 这种先进的技术揭示了以前从结构生物学中隐藏的细胞过程,
科学领域:
- 结构生物学
- 生物化学
- 细胞生物学
背景情况:
- 了解细胞功能需要对3D生物大分子结构的了解.
- 在研究细胞过程中的键形成/裂变时,原子层的结构细节至关重要.
研究的目的:
- 要突出冷电子显微镜对结构生物学的影响.
- 展示冷EM的潜力,以表征以前无法获得的细胞过程.
主要方法:
- 冷水合样本的电子显微镜的发展 (冷电子显微镜).
- 生物大分子的高分辨率结构特征.
主要成果:
- 电子显微镜为结构特征提供了前所未有的机会.
- 能够在原子层面详细研究生物大分子.
结论:
- 通过揭示复杂的细胞机制,
- 这种技术为我们了解细胞功能开辟了新的途径,
相关概念视频
01:28Cryo-electron Microscopy
4.5K
Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...
4.5K
01:07Electron Microscope Tomography and Single-particle Reconstruction
3.0K
Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
3.0K