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证据表明VEGF-Ax的益血管功能

Hong Xin¹, Cuiling Zhong¹, Eric Nudleman¹

¹University of California, San Diego, 3855 Health Sciences Drive #0819, La Jolla, CA 92093, USA.

|September 24, 2016

概括

与之前的发现相反,血管内皮生长因子-Ax (VEGF-Ax) 促进血管形成. 这项研究显示,VEGF-Ax刺激内皮细胞生长和血管透性,影响血管生成研究.

科学领域:

分子生物学
细胞生物学
生物化学

背景情况:

血管内皮生长因子A (VEGF-A) 异型对血管发育至关重要.
VEGF信号通路是各种疾病的有效治疗点.
替代mRNA拼接产生多个VEGF-A异型,例如VEGF165.

研究的目的:

研究VEGF-Ax异型在内皮细胞中的功能作用.
为了澄清关于VEGF-Ax的亲血管性或抗血管性作用的相互矛盾的报告.
确定VEGF-Ax对内皮细胞增殖,迁移和血管透性的影响.

主要方法:

细胞培养内皮细胞
对VEGF-Ax对细胞增殖 (甲基生成) 的影响的分析.
血管生成和血管透性测试的评估.
检测VEGFR-2酸化的西部抹杀.
使用neuropilin-1进行VEGF-Ax和VEGF165结合测定.

主要成果:

VEGF-Ax显著刺激了内皮细胞的线粒生成,血管生成和血管透性.
在VEGFR-2中,VEGF-Ax诱导了关键氨酸残留物的酸化.
与VEGF165相比,VEGF-Ax的功效有所降低.
观察到VEGF-Ax与神经素-1共受体的结合功能受损.

结论:

这与之前的研究相矛盾.
VEGF-Ax和VEGF165的作用差异部分是由于神经皮林-1结合的变化.
在涉及血管生成的治疗策略中,VEGF-Ax是一个新的点.

关键词:

一个 VEGF 血管新生细胞内膜通过翻译阅读氨酸激酶

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