功能代谢学描述了酵母生物合成规律
Michael Mülleder1, Enrica Calvani1, Mohammad Tauqeer Alam2
1Department of Biochemistry and Cambridge Systems Biology Centre, University of Cambridge, Cambridge CB2 1GA, UK; The Francis Crick Institute, Mill Hill Laboratory, Mill Hill, London NW7 1AA, UK.
Cell
|October 4, 2016
概括
这项研究绘制了酵母基因代谢相互作用图,揭示了超过三分之一的基因对生物合成产生影响. 代谢特征有助于识别未表征的基因的功能,并了解细胞调节.
科学领域:
- 分子生物学
- 系统生物学
- 代谢学
背景情况:
- 基因组代谢相互作用对细胞生长和功能至关重要.
- 了解这些相互作用是解读细胞调节网络的关键.
- 系统的基因删除研究是功能性基因组学的强大工具.
研究的目的:
- 量化Saccharomyces氨基酸代谢组及其对基因删除的反应.
- 确定基因对生物合成代谢的功能贡献.
- 利用代谢组数据对未表征的基因进行功能注释.
主要方法:
- 对Saccharomyces氨基酸代谢的量化
- 系统的基因删除分析以观察代谢反应.
- 功能代谢学,将代谢调节剂与它们的效应器联系起来.
主要成果:
- 超过三分之一的编码基因,特别是那些涉及染色体动力学,翻译和运输的基因,有助于生物合成代谢.
- 不同的氨基酸代谢特征与基因功能相关.
- 代谢特征为一半以上未表征的酵母基因提供功能性注释,提供超越遗传和物理相互作用网络的见解.
结论:
- 有很大一部分编码基因参与基因代谢相互作用,揭示了调节网络.
- 代谢分析提供了一种探索基因功能和细胞调节的新方法.
- 这项工作通过探索尚未探索的代谢空间来扩大对基因功能的理解.
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