丝虫PIWI-CladeArgonaute Siwi结合到piRNA的晶体结构
Naoki Matsumoto1, Hiroshi Nishimasu2, Kazuhiro Sakakibara1
1Graduate School of Science, Department of Biological Sciences, The University of Tokyo, 2-11-16 Yayoi, Bunkyo-ku, Tokyo 113-0032, Japan.
Cell
|October 4, 2016
概括
研究人员确定了与PIWI相互作用的RNA (piRNA) 结合的PIWI-clade Argonaute (Siwi) 的晶体结构. 这揭示了Siwi如何沉默动物性腺体中的可转移元素的关键结构见解.
科学领域:
- 分子生物学
- 结构生物学
- 遗传学
背景情况:
- 在动物生殖系中,PIWI-clade Argonaute 蛋白对于通过 PIWI 相互作用的 RNA (piRNA) 静止可移植元素至关重要.
- 了解PIWI-Argonaute-piRNA复合物的结构基础对于阐明转子子沉默机制至关重要.
研究的目的:
- 为了确定PIWI-clade的晶体结构,Siwi,与其内源的piRNA复合.
- 将Siwi与其他Argonaute蛋白质的结构进行比较,以确定与指导RNA结合和功能相关的保存和分离特征.
主要方法:
- 使用X射线晶体学以2.4 Å分辨率获得丝虫Siwi-piRNA复合物的晶体结构.
- 在确定Siwi结构和已知的AGO-cladeArgonaute蛋白质结构之间进行了结构比较.
主要成果:
- 晶体结构显示Siwi的双叶结构,具有明显的N-PAZ和MID-PIWI叶.
- 该piRNA的5'和3'端分别被MID-PIWI和PAZ域定.
- 与AGO-clade Argonauts相比,Siwi表现出独特的核酸结合通道特征,并且与原生细胞AGO-clade Argonauts有相似之处,包括金属依赖的5'-酸盐识别.
结论:
- Siwi-piRNA结构为理解PIWI介导的转子子沉默提供了详细的分子框架.
- 结构上的差异凸显了PIWI和AGO类别之间指导RNA处理的独特机制.
- 这项研究提供了关于PIWI Argonautes和 prokaryotic AGO-clade蛋白质之间的进化关系的见解.
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