斑马鱼幼虫尾骨中的矿物质形成通过酸性酸盐阶段发生
Anat Akiva1, Michael Kerschnitzki1, Iddo Pinkas2
1Department of Structural Biology, Weizmann Institute of Science , Rehovot 76100, Israel.
Journal of the American Chemical Society
|October 7, 2016
概括
骨形成过程中出现了前体阶段的失序. 斑马鱼尾骨的研究显示,骨细胞界面的酸性,HPO42-丰富的酸盐相异常,对骨矿化至关重要.
科学领域:
- 生物矿物化
- 骨生物学
- 结晶过程
背景情况:
- 骨矿物质形成的假设是涉及无序的前体阶段.
- 之前的研究检测到类似八酸,无形酸和HPO42-) 丰富的相.
- 这些前体阶段的确切性质和作用仍未完全确定.
研究的目的:
- 描述和绘制斑马鱼尾骨形成过程中的矿物阶段.
- 调查骨发育中的失序前体阶段的作用.
- 阐明早期骨矿产沉积物的化学和结构性质.
主要方法:
- 活体拉曼光谱分析斑马鱼幼虫中的矿物阶段.
- 高分辨率广角X射线衍射 (WAXD) 检查斑马鱼幼虫牺牲后的矿物相.
- 在尾骨结构中的矿物相的2μm分辨率映射.
主要成果:
- 拉曼光谱检测出一种酸性,无序的酸盐阶段,在骨细胞接口上有HPO42-的特征.
- WAXD检测到一个未识别的阶段, 不同于碳化酸, 在骨组织接口.
- 这种前体表现出可变的峰值位置,表明不稳定,并与成熟的骨晶体和原一起排列.
结论:
- 一个含有HPO4的显著无序矿物阶段是斑马鱼尾翼骨沉积途径的组成部分.
- 这些发现支持骨矿化失序的前体模型.
- 前体相与原蛋白的密切关联凸显了其在矩阵中介矿化中的作用.
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