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DNA基质的扭曲约束 冲击Cas9裂变

Michael H Räz1, Kumi Hidaka2, Shana J Sturla1

  • 1Department of Health Sciences and Technology, ETH Zürich , Schmelzbergstrasse 9, 8092 Zürich, Switzerland.

Journal of the American Chemical Society
|October 7, 2016
PubMed
概括

对DNA的扭曲约束显著影响了Cas9的裂变. 限制非目标链降低了Cas9的效率,而限制目标链的影响最小,揭示了DNA结构

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