Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

piRNA - Piwi-interacting RNAs02:57

piRNA - Piwi-interacting RNAs

7.8K
PIWI-interacting RNAs, or piRNAs, are the most abundant short non-coding RNAs. More than 20,000 genes have been found in humans that code for piRNAs while only 2000 genes have been found for miRNAs. piRNAs can act at the transcriptional and post-transcriptional levels and have a vital role in silencing transposable elements present in germ cells. They are also involved in epigenetic silencing and activation. Previously, they were thought to function only in germ cells but new evidence suggests...
7.8K
Cloning of Dolly the Sheep01:08

Cloning of Dolly the Sheep

8.5K
The first successfully cloned mammal was Dolly, a sheep, born on 5th July 1996 at Roslin Institute, Scotland. The cloned sheep was named after the American singer Dolly Parton. Dolly lived for seven years and died of respiratory complications, which is speculated to be due to the actual age of her DNA. Because the DNA in cloned cells belongs to an older individual,  the cloned individual’s life expectancy may be affected. Indeed, analysis of Dolly’s DNA revealed shorter...
8.5K
Piaget's Stage 1 of Cognitive Development01:14

Piaget's Stage 1 of Cognitive Development

2.2K
The sensorimotor stage, the initial phase of Jean Piaget's theory of cognitive development, spans the first two years of a child's life. During this period, infants actively engage with their surroundings, building cognitive awareness through direct interaction with the world. This interaction is primarily based on sensory perception and motor actions, allowing infants to gradually understand basic physical properties and predict how objects interact within their environment.
Exploration...
2.2K
Pinocytosis00:43

Pinocytosis

71.5K
Cells use energy-requiring bulk transport mechanisms to transfer large particles, or large amounts of small particles, into or out of the cell. The cells envelop the particles in spherical membranes called vesicles or vacuoles. Vesicles that transport material into the cell are built from the cell membrane. These vesicles encapsulate external molecules and transport them into the cell in a process called endocytosis.
71.5K
Pinocytosis00:38

Pinocytosis

5.0K
Cells use energy-requiring bulk transport mechanisms to transfer large particles or large numbers of small particles into or out of the cell. The cells envelop the particles in spherical membranes called vesicles or vacuoles. Vesicles that transport material into the cell are built from the cell membrane. These vesicles encapsulate external molecules and transport them into the cell in a process called endocytosis.
Pinocytosis ("cellular drinking") is one of three main types of...
5.0K
Binary Fission01:20

Binary Fission

66.2K
Fission is the division of a single entity into two or more parts, which regenerate into separate entities that resemble the original. Organisms in the Archaea and Bacteria domains reproduce using binary fission, in which a parent cell splits into two parts that can each grow to the size of the original parent cell. This asexual method of reproduction produces cells that are all genetically identical.
66.2K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Structure basis for single-strand nucleic acid targeting by IscB and variants.

Nucleic acids research·2026
Same author

Crystal structure of the cytosolic Nudix-like domain of CD-NTase-associated protein 16 from Enterococcus faecalis.

Acta crystallographica. Section F, Structural biology communications·2026
Same author

The antiphage mechanism of a widespread trypsin-MBL defense module.

Nature chemical biology·2026
Same author

Rational design of carbazole-based STING inhibitors for treating cGAS-STING pathway-driven inflammatory disorders.

Nature communications·2026
Same author

Structural and functional characterization of SPARTA system.

Biochemical and biophysical research communications·2026
Same author

Structure basis for single-strand nucleic acid targeting by IscB and variants.

bioRxiv : the preprint server for biology·2026

相关实验视频

Updated: Mar 14, 2026

A Noninvasive Hair Sampling Technique to Obtain High Quality DNA from Elusive Small Mammals
07:40

A Noninvasive Hair Sampling Technique to Obtain High Quality DNA from Elusive Small Mammals

Published on: March 13, 2011

21.5K

一个巨大的进步

Yibei Xiao1, Ailong Ke1

  • 1Department of Molecular Biology and Genetics, Cornell University, 251 Biotechnology Building, Ithaca, NY 14853, USA.

Cell
|October 8, 2016
PubMed
概括

称为piRNAs的小RNA指导PIWI蛋白在动物繁殖中使转子沉默. 研究人员揭示了与piRNA结合的PIWI蛋白质的第一个晶体结构,为这种沉默机制提供了新的见解.

科学领域:

  • 分子生物学
  • 遗传学
  • 结构生物学

背景情况:

  • 与PIWI相互作用的RNAs (piRNAs) 在动物生殖系中对可移植元素的沉默至关重要.
  • 由piRNA诱导的沉默复合体 (piRISC) 通过复杂的生物生成途径来调解这种沉默.
  • 了解PIWI蛋白功能的结构基础是阐明piRISC组合和活动的关键.

研究的目的:

  • 确定与指导RNA结合的PIWI-clade蛋白质的第一个晶体结构.
  • 提供关于piRISC复合体中PIWI蛋白的作用前状态的结构见解.

主要方法:

  • 使用X射线结晶学来获得高分辨率的结构.
  • 使用生物化学和生物物理技术来描述PIWI导向RNA复合体.

主要成果:

  • 晶体结构显示了一个PIWI蛋白质,
  • 该结构阐明了PIWI蛋白与指导piRNA之间的精确相互作用.

结论:

  • 这项结构研究提供了PIWI指导RNA相互作用的基础知识.
  • 这些发现为未来关于piRISC形成和功能机制的研究铺平了道路.

更多相关视频

Transition of Farm Pigs to Research Pigs using a Designated Checklist followed by Initiation of Clicker Training - a Refinement Initiative
07:59

Transition of Farm Pigs to Research Pigs using a Designated Checklist followed by Initiation of Clicker Training - a Refinement Initiative

Published on: August 21, 2021

6.4K
Recording Single Neurons' Action Potentials from Freely Moving Pigeons Across Three Stages of Learning
11:20

Recording Single Neurons' Action Potentials from Freely Moving Pigeons Across Three Stages of Learning

Published on: June 2, 2014

12.5K

相关实验视频

Last Updated: Mar 14, 2026

A Noninvasive Hair Sampling Technique to Obtain High Quality DNA from Elusive Small Mammals
07:40

A Noninvasive Hair Sampling Technique to Obtain High Quality DNA from Elusive Small Mammals

Published on: March 13, 2011

21.5K
Transition of Farm Pigs to Research Pigs using a Designated Checklist followed by Initiation of Clicker Training - a Refinement Initiative
07:59

Transition of Farm Pigs to Research Pigs using a Designated Checklist followed by Initiation of Clicker Training - a Refinement Initiative

Published on: August 21, 2021

6.4K
Recording Single Neurons' Action Potentials from Freely Moving Pigeons Across Three Stages of Learning
11:20

Recording Single Neurons' Action Potentials from Freely Moving Pigeons Across Three Stages of Learning

Published on: June 2, 2014

12.5K