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对于细胞静止来说,RNA干扰至关重要

B Roche¹, B Arcangioli², R A Martienssen³

¹Howard Hughes Medical Institute-Gordon and Betty Moore Foundation, Cold Spring Harbor Laboratory, 1 Bungtown Road, Cold Spring Harbor, NY 11724, USA.

Science (New York, N.Y.)

|October 15, 2016

概括

在分裂酵母中,RNA干扰 (RNAi) 对于细胞周期静止 (G0阶段) 是必不可少的. RNAi突变体表现出G0入口缺陷和降低活力,突出显示RNAi

科学领域:

细胞生物学
分子生物学
遗传学

背景情况:

静止细胞 (G0阶段) 对于生物的发育和生存至关重要.
对于RNA干扰 (RNAi) 在细胞周期静止的调节中的作用仍然在很大程度上未被探索.
了解细胞循环调节对于发育生物学和疾病研究至关重要.

研究的目的:

为了调查RNA干扰 (RNAi) 对于Schizosaccharomyces pombe细胞静止的要求.
确定与RNAi相关的G0阶段缺陷的遗传抑制剂.
阐明RNAi影响细胞周期进展和静止的分子机制.

主要方法:

基因查以确定Dicer (dcr1Δ) 突变体中的G0缺陷抑制剂.
在缺少RNAi的裂变酵母中分析细胞活力和细胞周期进展.
研究与RNA聚合酶活性相关的基因表达模式和染色质修饰.

主要成果:

在G0进入和长期静止期间,RNA干扰 (RNAi) 对于维持细胞活力至关重要.
缺乏Dicer (dcr1Δ) 的突变体在G0输入和维护方面表现出显著的缺陷.
抑制分析确定了参与染色体分离,RNA聚合酶调节和异染色体形成的基因.

结论:

通过调节RNA聚合酶释放,RNAi促进细胞周期静止.
通过RNA聚合酶II释放,可以在中间体上促进异染色体的形成,从而实现适当的染色体分离.
释放的RNA聚合酶I可以阻止核糖体DNA中的异染色体的形成,这对维持静态性至关重要.

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