热性古生物通过合酶M形成激活
Rafael Laso-Pérez1,2, Gunter Wegener1,2,3, Katrin Knittel1
1Max-Planck Institute for Marine Microbiology, 28359 Bremen, Germany.
Nature
|November 8, 2016
概括
科学家发现,古生物可以通过与甲相似的途径分解四碳化合物. 这一发现表明在地下环境中无氧化碳氧化存在广泛的自然机制.
科学领域:
- 微生物学
- 生物化学
- 地质化学
背景情况:
- 厌氧甲氧化 (AMO) 依赖于C1化合物的独特酶.
- 除了甲之外,无氧碳化合物氧化的酶机制和辅因子在很大程度上是未知的.
- 已知古生物在厌氧甲循环中起着关键作用.
研究的目的:
- 通过微生物团队对C4碳化合物的无氧氧化进行研究.
- 识别与丁氧化有关的微生物和酶途径.
- 确定甲氧化途径是否用于长链碳化合物.
主要方法:
- 用丰富的无氧热友群体的培养
- 考古和细菌伙伴的基因组和转录组分析.
- 生物化学分析以检测中间代谢物,如M酶.
主要成果:
- 鉴定出一种古老的物种",Candidatus Syntrophoarchaeum",表现出类似甲的自光和表达甲基辅酶M减少酶同类物质.
- 检测到布辅酶M,表明古生物激活了布,类似于甲激活.
- 考古物还表达了β-氧化和C1代谢的基因,使得完全氧化,并将降低剂转移到降低硫酸盐的细菌 (HotSeep-1).
结论:
- 古生物可以通过与甲相似的途径激活和氧化丁,其中包括丁共酶M.
- 这种机制将已知的无氧C1代谢能力扩展到C4碳化合物.
- 海洋地下沉积物中类似基因的流行表明这种丁氧化途径在自然界中广泛存在.
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