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MOF乙转移酶调节了线粒体中的转录和呼吸
Aindrila Chatterjee1, Janine Seyfferth2, Jacopo Lucci2
1Max Planck Institute of Immunobiology and Epigenetics, 79108 Freiburg, Germany; Faculty of Biology, Albert Ludwigs University of Freiburg, 79085 Freiburg, Germany.
Cell
|October 22, 2016
概括
在线粒体中发现的MYST相互作用蛋白MOF (男性特异性女性的致命脱轨),在核和线粒体DNA中调节细胞呼吸和基因表达. 它的缺失导致严重的心脏缺陷, 连接表观遗传与代谢.
科学领域:
- 分子生物学
- 表观遗传学
- 细胞代谢
背景情况:
- 表观遗传调节和代谢控制可以相互作用以适应细胞对环境变化的反应.
- MYST家族的乙转移酶MOF (男性特异性致死性脱轨的女性产生) 是一个已知的核蛋白.
研究的目的:
- 研究MOF在细胞代谢中的作用及其在细胞核外的潜在定位.
- 了解MOF对线粒体功能的影响及其与表观遗传调节的联系.
主要方法:
- 免疫光和生物化学测试以检测线粒体中的MOF局部化.
- 从核和线粒体DNA (mtDNA) 的基因表达分析.
- 在Mof条件淘汰小鼠中评估氧化化和心脏功能.
主要成果:
- MOF及其复杂伙伴在线粒体中局部存在.
- MOF通过控制核和mtDNA的呼吸基因表达来调节氧化酸化.
- MOF与mtDNA结合,依赖于KANSL3.
- 缺少MOF的小鼠表现出严重的心脏缺陷,包括缩性心肌病和线粒体退化.
结论:
- MOF 作为核和线粒体基因组的双重转录调节器.
- MOF将表观遗传机制与代谢控制联系起来,特别是在高能耗组织中.
- 线粒体MOF对于维持心脏功能和细胞呼吸至关重要.
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