呼吸综合体III和IV的超级组合机制
Sara Cogliati1, Enrique Calvo1, Marta Loureiro1
1Centro Nacional de Investigaciones Cardiovasculares Carlos III, 28029 Madrid, Spain.
Nature
|November 8, 2016
概括
超复杂组合因子1 (SCAF1) 对于呼吸链超复杂的形成至关重要. 较长的SCAF1异形使呼吸酶组合成为可能,而较短的异形则会在大多数组织中破坏这种组合.
科学领域:
- 线粒体生物学
- 蛋白质复合组件
- 细胞代谢
背景情况:
- 呼吸链复合体形成超级复合体,优化细胞代谢.
- 超复杂组合因子1 (SCAF1) 调节复合物III (CIII) 和IV (CIV) 之间的相互作用.
- SCAF1存在于长和短的异构形式,只有长形式诱导CIII-CIV超级组件.
研究的目的:
- 确定是否需要SCAF1来形成呼吸体 (CI,CIII2,CIV超复合体).
- 研究SCAF1异型在呼吸体组合中的作用.
- 确定SCAF1区域和特定的残留物对CIII和CIV相互作用至关重要.
主要方法:
- 深度蛋白质组学和免疫检测分析
- 针对SCAF1的突变发生.
- 在不同小鼠组织中分析SCAF1异型.
主要成果:
- 在大多数组织中,SCAF1对于CIII-CIV相互作用和呼吸酶形成至关重要.
- 短的SCAF1异型阻止呼吸酶组合由于改变的histidine在位置73.
- 在CIII-CIV超复合体中,SCAF1取代了COX7A2;在CIV二元体中发现了COX7A1.
结论:
- SCAF1对于呼吸体完整性至关重要,具有异形特异性的功能.
- 短SCAF1异型不能与CIV相互作用,因此阻碍了呼吸体的形成.
- 除心脏和骨肌肉外,SCAF1异型的组织特异表达影响呼吸体的存在.
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