生物活性两分子对的层次组合成超分子纳米纤维素自我支的支架,用于干细胞分化
Zhe Wang1, Fuwu Zhang1, Zhantong Wang1
1Laboratory of Molecular Imaging and Nanomedicine and ‡Laboratory of Cellular Imaging and Macromolecular Biophysics, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health , Bethesda, Maryland 20892, United States.
Journal of the American Chemical Society
|November 5, 2016
概括
研究人员创造了可自组装的生物材料,模仿细胞外基质 (ECM),以改善干细胞培养. 这些生物活性水凝支持神经干细胞的生长和分化,没有添加生长因子.
科学领域:
- 生物材料科学
- 干细胞生物学
- 组织工程
背景情况:
- 细胞外基质 (ECM) 提供了支持细胞功能的自然.
- ECM的层次结构和生物活性分子影响细胞行为.
- 模仿ECM对于促进干细胞培养和组织再生至关重要.
研究的目的:
- 设计和合成可以自组合成人工ECM的生物活性分子.
- 为增强干细胞培养创建自支持的纳米纤维水凝.
- 研究这些生物材料对神经干细胞分化的潜力.
主要方法:
- 合成生物模拟分子的等级自组成纳米纤维液凝.
- 使用静电相互作用,范德瓦尔斯力和键进行自组.
- 使用可降解的水凝在培养过程中促进细胞迁移和扩张.
主要成果:
- 生物模拟分子成功形成了自我支持的纳米纤维液体.
- 这些水凝显示出受控的降解,促进细胞迁移和扩张.
- 没有生长因子的支架加速了神经干细胞的粘附,增殖和分化.
结论:
- 生物活性分子可用于新生物材料的自组装.
- 模仿ECM的水凝为干细胞培养和组织工程提供了一个有前途的策略.
- 这种方法为设计影响细胞行为的先进生物材料提供了新的途径.
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