通过基因组重新编码来定义同义的子压缩方案
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在PubMed上查看摘要
概括
此摘要是机器生成的。科学家们开发了一种重写细菌基因组的新方法,使得合成DNA的创造成为可能. 这一突破使大肠杆菌的基因组重新编码成为可能,并定义了基因设计的规则.
科学领域
- 合成生物学
- 基因组学
- 分子生物学
背景情况
- 基因组的合成重新编码旨在使用直角转换系统来实现非自然聚合物的合成.
- 对同义代码子替代的理解有限,缺乏逐步基因组替代的方法阻碍了进展.
研究的目的
- 在大肠杆菌中有效,可编程地用长合成DNA替换基因组DNA的系统.
- 在合成基因组中建立允许和不允许的设计特征的反.
主要方法
- 利用CRISPR/Cas9在体内切除从一个副本的双链DNA.
- 采用兰巴红介导的重组,同时进行正负选择以逐步更换基因组.
- 将八种同名重编码方案应用于系统性基因组重编码的基本操作.
主要成果
- 通过长 (>100kb) 的合成DNA有效,可编程地替换基因组DNA的系统.
- 在大肠杆菌中逐步进行全基因组替代的基础.
- 定义了允许和不允许的同义重编码方案,并确定了特殊的重编码位置.
结论
- 开发的系统有助于高效和可编程的合成基因组工程.
- 这项工作为同义代码使用和基因组设计规则提供了关键的见解.
- 能够识别和修复未来合成基因组应用的重编码错误.
相关概念视频
The flow of genetic information in cells from DNA to mRNA to protein is described by the central dogma, which states that genes specify the sequence of mRNAs, which in turn specify the sequence of amino acids making up all proteins. The decoding of one molecule to another is performed by specific proteins and RNAs. Because the information stored in DNA is so central to cellular function, it makes intuitive sense that the cell would make mRNA copies of this information for protein synthesis...
Overview
The central dogma of biology states that information encoded in the DNA is transferred to messenger RNA (mRNA), which then directs the synthesis of protein. The set of instructions that enable the mRNA nucleotide sequence to be decoded into amino acids is called the genetic code. The universal nature of this genetic code has spurred advances in scientific research, agriculture, and medicine.
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The central dogma explains the flow of genetic information from DNA nucleotides to the amino acid sequence of proteins.
RNA is the Missing Link Between DNA and Proteins
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Exon shuffling follows “splice frame rules.” Each exon...
Other than maintaining genome stability via DNA repair, homologous recombination plays an important role in diversifying the genome. In fact, the recombination of sequences forms the molecular basis of genomic evolution. Random and non-random permutations of genomic sequences create a library of new amalgamated sequences. These newly formed genomes can determine the fitness and survival of cells. In bacteria, homologous and non-homologous types of recombination lead to the evolution of new...