细胞间的沟通和结合由真菌菌中的ESX分泌系统介导
Todd A Gray1,2, Ryan R Clark3, Nathalie Boucher3
1Wadsworth Center, New York State Department of Health, P.O. Box 22002, Albany, NY 12201-2002, USA. todd.gray@health.ny.gov keith.derbyshire@health.ny.gov.
概括
Mycobacterium smegmatis使用ESX-1分泌系统来控制ESX-4的表达,在细菌结合过程中促进细胞间通信和DNA转移.
科学领域:
- 微生物学
- 细菌遗传学
- 细胞间通信
背景情况:
- 细胞间的沟通对于细菌群体至关重要,通常由分泌系统介导.
- 这种沟通以前没有在真菌细菌中表现出来.
- 已知ESX分泌系统是细菌相互作用的调解者.
研究的目的:
- 为了研究 Mycobacterium smegmatis 的细胞间通信.
- 确定ESX-4分泌系统在真菌结合中的作用.
- 阐明ESX-1和ESX-4分泌系统之间的调节关系.
主要方法:
- 研究ESX-4在Mycobacterium smegmatis受体活动中的作用.
- 在不同的培养条件下分析了esx4基因的转录调节.
- 在捐赠者和接受者细胞中使用ESX-1分泌装置的突变菌株.
- 与配偶DNA转移效率相关的ESX-1活动.
主要成果:
- 在Mycobacterium smegmatis中,ESX-4对配偶受体活动至关重要.
- 转录esx4基因需要与捐赠菌株和功能ESX-1装置共同培养.
- 捐赠者ESX-1装置的突变会放大接受者的ESX4转录反应.
- 通过ESX-1介导的细胞间通信调节ESX-4的表达.
结论:
- 通过ESX-1分泌系统进行细胞间通信,控制其进化祖先ESX-4的表达.
- 这种调节促进了真菌菌之间的结合和DNA转移.
- ESX-1 作为ESX-4 的调节剂,突出显示了菌根体中的新型传播途径.
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