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相关概念视频

DNA Microarrays02:34

DNA Microarrays

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Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
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Epigenetic Regulation01:37

Epigenetic Regulation

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Epigenetic changes alter the physical structure of the DNA without changing the genetic sequence and often regulate whether genes are turned on or off. This regulation ensures that each cell produces only proteins necessary for its function. For example, proteins that promote bone growth are not produced in muscle cells. Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
X-chromosome...
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Epigenetic Regulation01:46

Epigenetic Regulation

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Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
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相关实验视频

Updated: Mar 11, 2026

Chromatin Immunoprecipitation Assay Using Micrococcal Nucleases in Mammalian Cells
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Chromatin Immunoprecipitation Assay Using Micrococcal Nucleases in Mammalian Cells

Published on: May 10, 2019

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快照:表观基因组测试

Martin Krzywinski1, Martin Hirst2

  • 1Canada's Michael Smith Genome Sciences Centre, BC Cancer Agency Research Centre, BC Cancer Agency, Vancouver, BC V5Z 1L3, Canada.

Cell
|November 19, 2016
PubMed
概括
此摘要是机器生成的。

本研究回顾了表观基因组分析的基本测序技术. 涵盖的关键方法包括二硫酸盐测序,染色体免疫沉测序,开放染色体测定和3D染色体捕获以获得全面的见解.

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Screening for Functional Non-coding Genetic Variants Using Electrophoretic Mobility Shift Assay EMSA and DNA-affinity Precipitation Assay DAPA
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An Integrated Platform for Genome-wide Mapping of Chromatin States Using High-throughput ChIP-sequencing in Tumor Tissues
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An Integrated Platform for Genome-wide Mapping of Chromatin States Using High-throughput ChIP-sequencing in Tumor Tissues

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相关实验视频

Last Updated: Mar 11, 2026

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科学领域:

  • 表观遗传学和基因组学
  • 分子生物学技术

背景情况:

  • 表观基因组在基因调节和细胞功能中起着至关重要的作用.
  • 了解表观遗传修饰需要先进的分析方法.

研究的目的:

  • 提供基于表观基因组分析的主要测序方法的概述.
  • 突出这些技术在现代生物研究中的实用性.

主要方法:

  • 用于DNA甲基化分析的二硫酸盐测序.
  • 蛋白质与DNA相互作用的染色体免疫沉测序 (ChIP-seq).
  • 测试用于确定开放的染色体区域 (例如ATAC-seq,DNase-seq).
  • 用于研究基因组结构的3D染色体捕获技术 (例如,Hi-C).

主要成果:

  • 基于测序的方法为表观遗传景观提供了高分辨率的洞察力.
  • 每种方法都提供了关于DNA修饰,基因质标记和染色质结构的补充信息.
  • 这些技术对于在各种生物环境中解剖基因调节至关重要.

结论:

  • 测序技术是全面表观基因组分析的必不可少的工具.
  • 这些方法的整合允许对表观遗传学调节有多方面的理解.
  • 测序的进步继续推动表观遗传学的发现.