在体内,多蛋白质复合体占用c-fos血清反应元素不受增长因子诱导的影响
R E Herrera1, P E Shaw, A Nordheim
1Zentrum für Molekulare Biologie Heidelberg, Universität Heidelberg, FRG.
Nature
|July 6, 1989
概括
这就是c-fos基因.
科学领域:
- 分子生物学分子生物学
- 基因规则 基因规则
- 细胞信号传递 细胞信号传递
背景情况:
- 人类c-fos原瘤基因通过外部信号的快速诱导取决于血清反应元件 (SRE).
- 两种蛋白质,血清反应因子 (p67SRF) 和p62,在体外与SRE结合.
- 这些蛋白与SRE之间的相互作用对于c-fos基因诱导至关重要.
研究的目的:
- 通过使用二甲基硫酸盐基因足迹在c-fos SRE中研究体内蛋白质相互作用.
- 为了确定人体A431细胞中SRE及其旁边区域内的蛋白质-DNA接触.
- 了解这些相互作用如何在基因激活和抑制过程中发生变化.
主要方法:
- 用二甲基硫酸盐基因足迹绘制图谱来绘制蛋白质结合部位.
- 对人类A431细胞中c-fos SRE和相邻序列上的蛋白-DNA接触的分析.
- 在表皮生长因子 (EGF) 诱导之前,期间和之后的蛋白质-DNA接触的比较.
主要成果:
- 基因组足迹揭示了与p67SRF,p62和p67SRF相一致的保护和超反应模式,以及一个额外的蛋白3'到p67SRF.
- 在EGF诱导之前,在SRE中观察到的蛋白质-DNA接触存在.
- 这些接触在基因激活和随后的抑制过程中保持不变.
结论:
- 在c-fos SRE存在稳定的DNA-蛋白结构,不管基因的转录状态如何.
- 这种在SRE中预先建立的结构可能在促进对细胞外信号的快速转录反应中发挥一般作用.
- 这些发现表明,通过保存的蛋白质-DNA相互作用来维持基因响应的机制.
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