对于应激反应转录的直接出口,绕过mRNA质量控制
Gesa Zander1, Alexandra Hackmann1, Lysann Bender1
1Abteilung für Molekulare Genetik, Institut für Mikrobiologie und Genetik, Göttinger Zentrum für Molekulare Biowissenschaften, Georg-August Universität Göttingen, Göttingen, Germany.
Nature
|December 13, 2016
概括
通过绕过适应蛋白和质量控制,细胞在压力时快速输出热冲击mRNA,确保生存. 正常的mRNA被保留在正常条件下维持细胞功能.
科学领域:
- 分子生物学
- 细胞生物学
- 遗传学
背景情况:
- 细胞需要特殊的生长和生存条件.
- 压力较大的环境需要快速生成保护性蛋白质.
- 细胞通过改变mRNA处理和输出来优先进行热冲击蛋白质合成.
研究的目的:
- 在细胞应激过程中阐明选择性mRNA输出机制.
- 了解细胞如何区分常规和热冲击mRNA用于出口.
- 确定RNA适应蛋白和Mex67-Mtr2在压力诱导的mRNA输出中的作用.
主要方法:
- 在Saccharomyces cerevisiae中研究了mRNA输出途径.
- 使用遗传和分子生物学技术研究蛋白质-RNA相互作用.
- 分析了热冲击转录因子Hsf1在应激基因表达中的作用.
主要成果:
- 细胞压力导致适应蛋白与正常mRNA分离,抑制它们的输出.
- 热冲击mRNAs被快速输出,直接与Mex67-Mtr2复合体结合,没有适应器.
- 热冲击mRNA出口绕过了适用于常规mRNA的严格质量控制机制.
- 转录因子Hsf1促进Mex67直接转录到热冲击mRNA.
结论:
- 细胞对mRNA出口采用双重策略:对常规mRNA进行质量控制的出口,以及对应激反应的mRNA进行快速,不受控制的出口.
- 这种选择性出口机制可以立即合成保护性蛋白质,
- 一个mRNA的命运 (质量控制或直接出口) 是由其促进器决定的,突出显示了应激反应的调节开关.
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