表面受体的封闭和随之而来的皮层张力是由常规的肌素产生的
C Pasternak1, J A Spudich, E L Elson
1Department of Cell Biology, Stanford University School of Medicine, California 94305.
Nature
|October 12, 1989
概括
传统的肌肉蛋白对于Dictyostelium细胞来说是必不可少的,它可以使表面蛋白质变硬和封闭. 没有肌素,细胞无法产生这些过程所需的皮质张力,影响细胞形状和受体封闭.
科学领域:
- 细胞生物学 细胞生物学
- 生物物理学的生物物理.
背景情况:
- 细胞骨收缩在各种细胞过程中起着至关重要的作用.
- 传统的肌肉蛋白是一种关键的运动蛋白,参与细胞力学.
研究的目的:
- 研究细胞骨收缩在表面蛋白质封闭中的作用.
- 确定对于皮层张力发育和细胞形状变化需要常规肌肉素的必要性.
主要方法:
- 测量细胞变形性,以评估皮质张力.
- 使用野生型 (AX4) 和突变型 (mhcA-) Dictyostelium 细胞.
- 诱导通过通过ATP耗尽与阿齐德强度收缩.
主要成果:
- 野生类型的细胞在封闭过程中变硬,然后放松.
- 缺少常规肌肉素的突变细胞表现出降低了性,并且无法封闭蛋白质.
- 在野生类型细胞中,ATP的耗尽会导致刚性收缩和硬化,但在突变细胞中并非如此.
结论:
- 传统的肌肉蛋白对于表面蛋白质的封闭和细胞硬度的增加都至关重要.
- 肌产生的收缩张力驱动细胞形状的变化和非肌肉细胞的受体封闭.
更多相关视频
08:50The Mechanics of (Poro-)Elastic Contractile Actomyosin Networks As a Model System of the Cell Cytoskeleton
Published on: March 10, 2023
06:48Tuning the Contractility and Deformation Modes of Active Actin-Based Assemblies In Vitro: From Two-Dimensional Active Networks to Liquid Crystal Drops
Published on: July 11, 2025
相关概念视频
Muscle Contraction
Actin and Myosin in Muscle Contraction
Actin and myosin are contractile proteins that form the sarcomere found in skeletal muscle tissues for regulating muscle contraction. Actin, a globular contractile protein, interacts with myosin for muscle contraction. The skeletal tissue appears striped or striated under a microscope due to the repeated arrangement of contractile proteins actin and myosin along the length of myofibrils. Dark A bands and light I bands repeat along myofibrils, and the alignment of myofibrils in the cell causes...
Muscle Contraction
In skeletal muscles, acetylcholine is released by nerve terminals at the motor endplate—the point of synaptic communication between motor neurons and muscle fibers. The binding of acetylcholine to its receptors on the sarcolemma allows entry of sodium ions into the cell and triggers an action potential in the muscle cell. Thus, electrical signals from the brain are transmitted to the muscle. Subsequently, the enzyme acetylcholinesterase breaks down acetylcholine to prevent excessive muscle...
Cell Motility through Blebbing
Blebs are a type of membrane protrusion formed by the internal hydrostatic pressure of the cytoplasm. Blebs are observed in several cell types, including fibroblasts, immune cells, and single-celled organisms like the amoeba. The primary function of blebs is cell locomotion and apoptosis, but they are also found during necrosis and cell division. The life cycle of a bleb comprises an initiation phase followed by the expansion and retraction phases.
Blebbing Through the Matrix
In multicellular...
Blebbing Through the Matrix
In multicellular...
Excitation-Contraction Coupling in Skeletal Muscles
Excitation-contraction coupling is a series of events that occur between generating an action potential and initiating a muscle contraction. It occurs at the triad, a structure found in skeletal muscle fibers that comprise a T-tubule and terminal cisternae of the sarcoplasmic reticulum on each side. These triads are visible in longitudinally sectioned muscle fibers. They are typically located at the A-I junction — the junction between the A and I bands of the sarcomere.
When an action potential...
When an action potential...
Relaxation of Skeletal Muscles
The period of muscle contraction primarily influences the duration of stimulation at the neuromuscular junction (NMJ), the presence of free calcium ions in the sarcoplasm, and the availability of energy or ATP to support contractions.
When an action potential reaches the axon terminal, it depolarizes the membrane and opens voltage-gated sodium channels. Sodium ions enter the cell, further depolarizing the presynaptic membrane. This depolarization causes voltage-gated calcium channels to open.
When an action potential reaches the axon terminal, it depolarizes the membrane and opens voltage-gated sodium channels. Sodium ions enter the cell, further depolarizing the presynaptic membrane. This depolarization causes voltage-gated calcium channels to open.
