在水中选择性识别单糖和寡糖的一般方法
Roshan W Gunasekara1, Yan Zhao1
1Department of Chemistry, Iowa State University , Ames, Iowa 50011-3111, United States.
Journal of the American Chemical Society
|December 17, 2016
概括
研究人员使用共价印记开发了纳米粒子受体来识别各种碳水化合物. 这些合成受体对d-aldohexoses具有很高的选择性,可以区分水中的葡萄糖,甘和银河糖.
科学领域:
- 碳水化合物化学
- 超分子化学
- 纳米技术
背景情况:
- 碳水化合物的分子识别在生物过程中至关重要.
- 开发合成碳水化合物受体面临重大挑战.
- 现有的方法往往缺乏所需的特异性和亲和力.
研究的目的:
- 为选择性碳水化合物识别创造新的纳米粒子受体.
- 研究醇功能化纳米颗粒与各种糖的结合能力.
- 为了实现生物相关单糖和寡糖的高选择性.
主要方法:
- 在boroxol功能化交叉连接小胞体内使用对碳水化合物的共价印记.
- 合成的蛋白质大小纳米粒子受体.
- 使用醇功能单体通过二醇相互作用结合糖模板.
- 在水溶液中评估结合性和选择性.
主要成果:
- 开发出能够识别广泛的单糖和寡糖的纳米粒子受体.
- 对d-aldohexoses表现出极好的选择性,区分葡萄糖,曼诺糖和银河糖.
- 在水中达到亚毫米度结合亲和度.
- 通过恐水相互作用增强结合.
- 寡糖的识别受到构建块,链接,长度和功能组的影响.
结论:
- 在醇功能化细胞中进行共价印记是一种有效的制造碳水化合物结合纳米粒子的策略.
- 这些纳米颗粒在水性介质中对d-aldohexoses具有很高的选择性和亲和力.
- 开发的受体为碳水化合物分析和传感应用提供了有前途的工具.
更多相关视频
11:49Sequencing of Plant Wall Heteroxylans Using Enzymic, Chemical Methylation and Physical Mass Spectrometry, Nuclear Magnetic Resonance Techniques
Published on: March 24, 2016
8.1K
05:19Author Spotlight: Advancing Protein Glycosylation Research Using a Fully Automated System
Published on: June 28, 2024
1.5K
相关概念视频
Oligosaccharide Assembly
3.8K
Protein glycosylation starts in the ER lumen and continues in the Golgi apparatus. Glycosyltransferases catalyze the addition of sugar molecules or glycosylation of proteins. Usually, these enzymes add sugars to the hydroxyl groups of selected serine or threonine residues to form O-linked glycans or the amino groups of asparagine residues to form N-linked glycans. Different positions on the same polypeptide chain can contain differently linked glycans.
Multiple sugar molecules that may or may...
Multiple sugar molecules that may or may...
3.8K
Carbohydrate Absorption
7.1K
Carbohydrates are essential macronutrients that serve as the body's primary energy source. Their digestion begins in the mouth, where salivary amylase partially breaks down complex carbohydrates such as starch into smaller oligosaccharides. This mechanical and enzymatic activity prepares carbohydrates for further processing in the gastrointestinal tract.
After being swallowed, the partially digested carbohydrates mix with gastric secretions in the stomach. However, the acidic environment...
After being swallowed, the partially digested carbohydrates mix with gastric secretions in the stomach. However, the acidic environment...
7.1K
Chemistry of Carbohydrates
92.4K
Carbohydrates are an essential part of the diet in humans and animals. Grains, fruits, and vegetables are natural sources of carbohydrates that provide energy to the body, particularly through glucose, a simple sugar that is a component of starch and an ingredient in many staple foods. The stoichiometric formula (CH2O)n, where n is the number of carbons in the molecule represents carbohydrates. In other words, the ratio of carbon to hydrogen to oxygen is 1:2:1 in carbohydrate molecules. This...
92.4K
Introduction to Carbohydrates
23.2K
Carbohydrates, proteins, and fats are the primary macronutrients in the human diet. However, carbohydrates are the most favored source of energy in the body. They can be found in a wide variety of foods, including whole grains, fruit, and vegetables, in various forms, such as sugars, starch, and dietary fiber. Based on their structure, carbohydrates are classified into three main classes— monosaccharides, disaccharides, and polysaccharides. The body's cells can only utilize simple...
23.2K
What are Carbohydrates?
202.3K
Overview
202.3K
Membrane Carbohydrates
7.6K
The plasma membrane is a dynamic barrier composed of lipids, proteins, and carbohydrates. It is the epicenter of many cellular processes required for cell growth and survival. Carbohydrates have unique structural and chemical properties that help the plasma membrane to carry out its functions effectively.
Membrane carbohydrates do not have any hydrophobic region and are exclusively located on the cell's outer surface. The addition of sugar molecules or glycosylation of proteins happens in...
Membrane carbohydrates do not have any hydrophobic region and are exclusively located on the cell's outer surface. The addition of sugar molecules or glycosylation of proteins happens in...
7.6K
