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相关概念视频

RNA Structure01:23

RNA Structure

79.8K
Overview
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...
79.8K
RNA Structure01:19

RNA Structure

8.0K
The basic structure of RNA consists of a string of ribonucleotides attached by phosphodiester bonds. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA) involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three...
8.0K
Nucleic Acid Structure01:25

Nucleic Acid Structure

9.8K
The pentose sugar in DNA is deoxyribose, while in RNA the pentose sugar is ribose. The difference between the sugars is the presence of the hydroxyl group on the ribose's second carbon and a hydrogen on the deoxyribose's second carbon. The phosphate residue attaches to the hydroxyl group of the 5′ carbon of one sugar and the hydroxyl group of the 3′ carbon of the sugar of the next nucleotide, which forms  a 5′ to 3′ phosphodiester linkage.
DNA Structure
DNA...
9.8K
Nucleic Acids02:43

Nucleic Acids

51.4K
Nucleic acids are the most important macromolecules for the continuity of life. They carry the cell's genetic blueprint and carry instructions for its functioning.
DNA and RNA
The two main types of nucleic acids are deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). DNA is the genetic material in all living organisms, ranging from single-celled bacteria to multicellular mammals. It is in the nucleus of eukaryotes and in the organelles, chloroplasts, and mitochondria. In prokaryotes,...
51.4K
Nucleic acids02:43

Nucleic acids

196.0K
Nucleic acids are the most important macromolecules for the continuity of life. They carry the cell's genetic blueprint and carry instructions for its functioning.
DNA and RNA
The two main types of nucleic acids are deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). DNA is the genetic material in all living organisms, ranging from single-celled bacteria to multicellular mammals. It is in the nucleus of eukaryotes and in the organelles, chloroplasts, and mitochondria. In prokaryotes,...
196.0K
RNA Interference01:23

RNA Interference

28.4K
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
28.4K

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相关实验视频

Updated: Mar 9, 2026

Probing RNA Structure with Dimethyl Sulfate Mutational Profiling with Sequencing In Vitro and in Cells
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Probing RNA Structure with Dimethyl Sulfate Mutational Profiling with Sequencing In Vitro and in Cells

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基于小分子的模式识别来分类RNA结构

Christopher S Eubanks1, Jordan E Forte1, Gary J Kapral1

  • 1Department of Chemistry, Duke University , Durham, North Carolina 27708, United States.

Journal of the American Chemical Society
|December 23, 2016
PubMed
概括
此摘要是机器生成的。

氨基糖类可以通过分析它们的结合模式来对RNA结构进行分类. 这种方法可以准确地预测RNA的二次结构,并揭示分子识别的关键因素.

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RNA Secondary Structure Prediction Using High-throughput SHAPE
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RNA Secondary Structure Prediction Using High-throughput SHAPE

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相关实验视频

Last Updated: Mar 9, 2026

Probing RNA Structure with Dimethyl Sulfate Mutational Profiling with Sequencing In Vitro and in Cells
10:34

Probing RNA Structure with Dimethyl Sulfate Mutational Profiling with Sequencing In Vitro and in Cells

Published on: December 9, 2022

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RNA Secondary Structure Prediction Using High-throughput SHAPE
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RNA Secondary Structure Prediction Using High-throughput SHAPE

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Sample Preparation for Mass Spectrometry-based Identification of RNA-binding Regions
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Sample Preparation for Mass Spectrometry-based Identification of RNA-binding Regions

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科学领域:

  • 生物化学
  • 结构生物学
  • 分子生物学

背景情况:

  • 确定三维RNA结构是一项挑战.
  • 了解RNA二次结构在构造中的作用是有限的.
  • 小分子:RNA识别的指导原则尚未得到充分确立.

研究的目的:

  • 开发一种用于分类RNA二次结构的方法.
  • 作为RNA分类的手段,研究氨基糖体结合.
  • 识别控制小分子的因素:RNA识别.

主要方法:

  • 使用主要成分分析 (PCA) 来分类五种正规的RNA二次结构图案.
  • 采用氨基糖化物作为受体和黄标记RNA作为分析物.
  • 含有详尽的关氨基基化物,以增强预测能力.

主要成果:

  • 在RNA训练组中实现100%的预测能力.
  • 使用生物相关结构的验证PCA,包括HIV-1 TAR RNA.
  • 确定了二次结构图案的核酸特异分类.
  • 揭示了氨基糖体:RNA识别的趋势,强调了形状,大小和序列的区别.

结论:

  • 开发了一种基于分子识别的RNA结构分类的新方法.
  • 证明RNA拓对于分子识别以及序列是至关重要的.
  • 为药物发现和RNA向提供了关于氨基糖胺:RNA相互作用的见解.