在生物粒子中绘制纳米表面的分子结构
Luciana M Herda1, Delyan R Hristov1, Maria Cristina Lo Giudice1
1Centre for BioNano Interactions, School of Chemistry, University College Dublin , Belfield, Dublin 4, Ireland.
Journal of the American Chemical Society
|December 23, 2016
概括
确定纳米粒子上的蛋白质定向是很困难的. 一种新的方法表明,在纳米颗粒上植入的蛋白质中,只有很小一部分是有利于细胞受体结合的.
科学领域:
- 纳米技术
- 生物结合化学
- 材料科学
背景情况:
- 鉴定纳米颗粒上的蛋白质定向对于有效的体外和体内向至关重要.
- 仅仅通过物理化学表征是不够的;纳米界面的生物学表征对于纳米材料的选择至关重要.
- 了解蛋白质定向影响纳米粒子生物功能和细胞受体参与.
研究的目的:
- 开发和验证一种评估纳米粒子表面蛋白质定向的方法.
- 基于蛋白质定位和表位可访问性来预测纳米粒子的生物功能.
- 评估移植到纳米颗粒上的转移蛋白的方向.
主要方法:
- 使用与金纳米颗粒 (免疫金) 结合的抗体碎片 (Fab) 作为探针.
- 在转移素移植的纳米颗粒 (SiO2-PEG8-Tf) 上绘制可用的表位.
- 使用免疫黄金标签来评估转移素表位素的可访问性,以识别受体.
主要成果:
- 该研究成功地绘制了转移素移植的纳米粒子表面上的可用表位.
- 数据表明,平均而言,只有大约3.5%的移植蛋白质表现出有利的方向.
- 这种有限的有利方向表明细胞受体参与的可访问性受到限制.
结论:
- 开发的方法提供了基于蛋白质定向的纳米粒子生物功能的预测.
- 纳米颗粒表面的显著比例的转移蛋白不是最佳的受体结合方向.
- 这一发现对生物系统中准纳米颗粒的设计和应用有影响.
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