一个三维电影的结构变化在bacteriorhodopsin
Eriko Nango1,2, Antoine Royant3,4, Minoru Kubo1,5
1RIKEN SPring-8 Center, 1-1-1 Kouto, Sayo-cho, Sayo-gun, Hyogo 679-5148, Japan.
Science (New York, N.Y.)
|December 24, 2016
概括
一个质子,在光激活后发生结构变化. 通过使用先进的X射线晶体学可视化这些运动,
科学领域:
- 膜生物物理
- 结构生物学
- 蛋白质动力学
背景情况:
- 细菌素 (bR) 作为光驱动的质子.
- 它可以作为膜运输蛋白的模型.
- 了解bR的机制是膜蛋白功能的关键.
研究的目的:
- 在光激活后可视化bacteriorhodopsin的构造变化.
- 阐明 bR 中质子运输的结构动力学.
- 从纳秒到毫秒的事件顺序.
主要方法:
- 时间分辨率的连续秒晶体学.
- 使用X射线自由电子激光器 (XFEL).
- 在高时间分辨率下捕获结构快照.
主要成果:
- 光激活导致视网膜染色体扭曲.
- 这种扭曲会取代基和水分子.
- 在蛋白质中观察到一连串的结构变化.
结论:
- 这项研究揭示了bR中质子运输的动作.
- 结构上的变化促进了质子在梯度上升的转移.
- 提供了对基本生物过程的原子层次洞察力.
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