人类雌激素受体有两个独立的非酸性转录激活功能
1Laboratoire de Génétique Moléculaire des Eucaryotes du CNRS, Unité 184 de Biologie Moléculaire et de Génie Génétique de l'INSERM Institut de Chimie Biologique, Faculté de Médecine, Strasbourg, France.
Cell
|November 3, 1989
概括
雌激素受体 (ER) 有两个转录激活功能:TAF-1在N端区域和TAF-2在激素结合域. 与酸性激活域不同,TAF-1 显示细胞特异性活性.
科学领域:
- 分子生物学分子生物学
- 内分泌学 在内分泌学.
- 基因法规 基因法规
背景情况:
- 雌激素受体 (ER) 在基因调节中起着至关重要的作用.
- 之前已经确定了在ER的激素结合域中激素诱导的转录激活功能 (TAF-2).
研究的目的:
- 对于转录激活功能的ER的N终端A/B区域的特征.
- 为了比较TAF-1与TAF-2以及已知的酸性激活域 (AADs) 的特性.
主要方法:
- 在不同细胞类型 (胚胎纤维细胞和HeLa细胞) 中,ER的N端区域 (TAF-1) 转录激活的分析.
- 评估TAF-1,TAF-2,GAL4和VP16AAD之间的协同作用.
- 调查与腺病毒2主要晚期促进体的上游元素 (UE) 因子的相互作用.
主要成果:
- 在ER的N终端A/B区域中确定了一个构成性激活函数 (TAF-1).
- TAF-1表现出细胞类型的特异性,在胚胎纤维细胞中比HeLa细胞更好地发挥作用.
- 与缺乏酸性氨基酸延伸的酸性激活域 (AADs) 相比,TAF-1和TAF-2具有不同的激活特性.
结论:
- 雌激素受体具有至少两个不同的转录激活功能:TAF-1 (构成性,细胞特异性) 和TAF-2 (可诱导性).
- 在机理上,TAF-1和TAF-2不同于正规的酸性激活域.
- 这些发现有助于理解雌激素受体介导基因调节的复杂机制.
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