在活细胞中可视化菌根膜动态
Frances P Rodriguez-Rivera1,2, Xiaoxue Zhou, Julie A Theriot3
1Department of Chemistry, University of California , Berkeley, California 94720, United States.
Journal of the American Chemical Society
|January 12, 2017
概括
研究人员开发了光探针来研究菌菌膜动力学. 他们发现膜流动性因物种而异,并随着乙胺醇治疗而增加,影响结核病药物开发.
科学领域:
- 微生物学
- 细胞生物学
- 生物化学
背景情况:
- 菌根菌具有强大的菌膜,有助于抗生素耐药性.
- 在这种膜内,糖脂的组织和动态尚不清楚.
研究的目的:
- 为了研究菌菌膜中糖脂的动态.
- 探索不同类型的菌膜流动性如何变化.
- 评估乙醇对菌膜动态的影响.
主要方法:
- 开发和应用三糖-酸联体,用于标记活类菌体中的三糖脂.
- 素-三醇类似物在三醇菌酸盐中的代谢结合.
- 时间间隔显微镜和光漂白后光恢复 (FRAP) 来研究糖脂的移动性.
主要成果:
- 光探针成功地被纳入了Mycobacterium,Corynebacterium,Nocardia和Rhodococcus物种的三菌.
- 菌膜流动性显示出显著的物种间变化,与酸结构相关.
- 使用结核病药物乙胺醇的治疗显著增加了真菌膜流动性.
结论:
- 三糖-合物为研究菌膜动力学提供了宝贵的工具.
- 菌膜流动性是一个可变的特征,受糖脂结构和药物治疗的影响.
- 这些发现提供了对菌根细胞外结构的见解,并提出了开发结核病药物的潜在策略.
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