在活细菌群中实时选生物催化剂
Cunyu Yan1, Fabio Parmeggiani1, Emrys A Jones2
1Manchester Synthetic Biology Research Centre for Fine and Speciality Chemicals (SYNBIOCHEM), Manchester Institute of Biotechnology, School of Chemistry, The University of Manchester , 131 Princess Street, M1 7DN, Manchester, United Kingdom.
Journal of the American Chemical Society
|January 14, 2017
概括
这项研究引入了一种新的无标签查平台,用于检测活细菌群中的生物催化活性. 该方法使用脱电喷射电离与离子移动性质谱成像 (DiBT-IMMS) 进行高效的酶发现.
科学领域:
- 生物技术
- 生物催化
- 分析化学
背景情况:
- 生物催化活动的传统选方法依赖于色度测量或基于标签的检测,限制了吞吐量和范围.
- 从细菌群体中识别新型酶对于生物技术和合成生物学的进步至关重要.
研究的目的:
- 开发和展示一个无标签的查平台,用于识别活体细菌群中的生物转化活动.
- 能够同时选多个酶变体和基质库.
主要方法:
- 使用溶解电喷离与离子移动性质谱成像 (DiBT-IMMS).
- 应用该平台对活细菌群中的氨酶和P450单氧酶进行查.
主要成果:
- 在活体细菌群中成功展示了无标签查方法.
- 能够同时使用DiBT-IMMS对酶变体和基质库进行多重选.
结论:
- DiBT-IMMS提供了一种强大的,无标签的方法,用于在活细菌群中对生物催化活动进行高吞吐量查.
- 这种平台通过克服传统查技术的局限性来推进酶发现和工程.
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