罗斯肉瘤病毒囊蛋白管状组合的结构模型
Jaekyun Jeon1, Xin Qiao1, Ivan Hung2
1Department of Physics, University of Central Florida , Orlando, Florida 32816, United States.
Journal of the American Chemical Society
|January 18, 2017
概括
对Rous瘤病毒 (RSV) 囊蛋白 (CA) 组合的结构洞察力揭示了域重构如何驱动囊曲线. 这项研究提供了RSV CA六合体管的详细原子模型,对于理解ortoretroviral囊形成至关重要.
科学领域:
- 结构生物学
- 病毒学
- 生物物理
背景情况:
- 甲状病毒体蛋白 (CA) 形成多态体,其结构,组合和稳定性仍然是积极研究的领域.
- CA由N终端 (NTD) 和C终端域 (CTD) 组成,它们调解同型和异型相互作用,在体表面形成六边形塔.
研究的目的:
- 确定组装到六合管中的罗斯肉瘤病毒 (RSV) CA的近完整固态NMR (ssNMR) 共振分配.
- 建立RSV CA管状组件的原子分辨率模型并阐明驱动曲的机制.
主要方法:
- 固态NMR (ssNMR) 用于组装的RSV CA六合管的共振分配.
- 用于结构约束的冷电子显微镜 (冷EM).
- 分子动力学灵活拟合 (MDFF) 模拟以构建原子分辨率模型.
主要成果:
- 在组装时,ssNMR赋值显示了循环和CTD启动螺旋的显著构造变化,具有静态无序的域间链接器.
- MDFF模型与ssNMR和冷EM数据相结合,表明状曲率来自重新配置的NTD-CTD和CTD形接口.
- 在CTD二元和三元接口的变化,以及普遍的H键,有助于CA六元格的可塑性和变形.
结论:
- 在RSV中,状曲线主要由域间接口的重新配置引起,由CTD在NTD六边形塔周围的可变位移驱动.
- 艾滋病毒和RSVCA组件之间的螺旋接触角度差异表明有不同的状组件路径.
相关概念视频
Protein Complex Assembly
17.0K
Proteins can form homomeric complexes with another unit of the same protein or heteromeric complexes with different types. Most protein complexes self-assemble spontaneously via ordered pathways, while some proteins need assembly factors that guide their proper assembly. Despite the crowded intracellular environment, proteins usually interact with their correct partners and form functional complexes.
Many viruses self-assemble into a fully functional unit using the infected host cell to...
Many viruses self-assemble into a fully functional unit using the infected host cell to...
17.0K
Protein Complex Assembly
2.6K
2.6K
Coat Assembly and GTPases
4.6K
Vesicles incorporate different coat protein subunits in different cell locations, which changes the properties of the coat, such as the shape and geometry of the transport vesicles. Thus, vesicle coat proteins also play a significant role in cargo selection.
Coat assembly depends on the local availability of phosphatidylinositol phosphates or PIPs and GTP-binding proteins. Adaptor proteins, which link the coat proteins to the membrane, bind to these PIPs and play a crucial role in controlling...
Coat assembly depends on the local availability of phosphatidylinositol phosphates or PIPs and GTP-binding proteins. Adaptor proteins, which link the coat proteins to the membrane, bind to these PIPs and play a crucial role in controlling...
4.6K
Assembly of Cytoskeletal Filaments
28.1K
Cytoskeletal filaments are polymeric forms of smaller protein subunits. However, individual cytoskeletal filaments may easily disassemble or associate with other similar filaments to form rigid structures. Microfilaments, made of actin monomers, rely on actin-binding proteins to form bundles and create networks of individual actin filaments. Microtubules rely on microtubule-associated proteins (MAPs) to form sturdy cylindrical structures. However, the proteins involved in forming complex...
28.1K
Rous Sarcoma Virus (RSV) and Cancer
6.5K
Rous Sarcoma virus or RSV was discovered by F. Peyton Rous in the year 1911 as a filterable transmissible agent that could cause tumors in chickens. He won a Nobel Prize for this discovery in 1966. His experiments clearly demonstrated that some cancers could be caused by infectious agents and led to the discovery of many more cancer-causing viruses in animals as well as humans.
RSV is a retrovirus that contains two copies of a plus-strand RNA genome. Its genome consists of four main open...
RSV is a retrovirus that contains two copies of a plus-strand RNA genome. Its genome consists of four main open...
6.5K
Viral Structure
75.4K
Viruses are extraordinarily diverse in shape and size, but they all have several structural features in common. All viruses have a core that contains a DNA- or RNA-based genome. The core is surrounded by a protective coat of proteins called the capsid. The capsid is composed of subunits called capsomeres. The capsid and genome-containing core are together known as the nucleocapsid.
75.4K


