通过与SGN3受体结合的血管衍生来控制根扩散屏障
Verónica G Doblas1, Elwira Smakowska-Luzan2, Satoshi Fujita1
1Department of Plant Molecular Biology, University of Lausanne, 1015 Lausanne, Switzerland.
Science (New York, N.Y.)
|January 21, 2017
概括
研究人员发现卡斯帕尔带完整性因子 (CIF1/2) 是SCHENGEN3类似受体激酶的配体. 这种相互作用对于保持根内皮扩散屏障至关重要,确保正确的卡斯帕带形成.
科学领域:
- 植物生物学
- 细胞生物学
- 分子遗传学
背景情况:
- 根内皮通过使用卡斯帕条形成了重要的扩散屏障.
- 众所周知,类似受体的激酶SCHENGEN3/GASSHO1 (SGN3/GSO1) 对卡斯帕带的形成和完整性至关重要.
- 调节SGN3功能的特定分子机制和配体在很大程度上是未知的.
研究的目的:
- 确定调节SCHENGEN3 (SGN3) 类似受体激酶功能的分子因子和配体.
- 阐明SGN3在根内皮中建立和维持卡斯帕带扩散屏障的机制.
- 调查硫化在卡斯帕尔带发育中的作用.
主要方法:
- 对影响硫化的申根2 (sgn2) 突变的基因分析.
- 标识和表达的特征,称为CASPARIAN STRIP INTEGRITY FACTORS (CIF1/2).
- 生物化学试验以证明直接与重组SGN3结合.
- 野生类型和突变植物中卡斯帕尔带形成和完整性的表型分析.
主要成果:
- 申根2 (sgn2) 突变体在负责硫化配体的酶中存在缺陷.
- 确定了两个表达的,CIF1和CIF2,作为SGN3的功能配体,在纳米度下补充sgn2突变物.
- 当外源性应用时,CIF1/ 2会以SGN3依赖的方式诱导卡斯帕带的错位化和过度线性化.
- 直接结合测定证实CIF1/2是SGN3的配体,这表明存在障碍监测系统.
结论:
- CIF1/ 2作为SGN3类似受体的配体,形成卡斯帕尔带完整性必不可少的信号模块.
- 建议CIF1/2-SGN3通道成为监控系统的关键组成部分,确保细胞上卡斯帕尔带网络的适当密封.
- 这一发现揭示了调节植物根的主要扩散屏障的分子机制.
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