通过可切换光发光来进行细胞内流通的宏分子""碳纳米粒子
Santosh K Misra1,2, Indrajit Srivastava1,2, Indu Tripathi1,2
1Departments of Bioengineering, Beckman Institute, Materials Science and Engineering, Institute for Sustainability in Energy and Environment, University of Illinois at Urbana-Champaign , Urbana, Illinois 61801, United States.
Journal of the American Chemical Society
|January 21, 2017
概括
研究人员开发了可开关的碳纳米粒子 (CNPs). 这一突破使得使用发光CNP进行生物成像的有效细胞内追踪成为可能.
科学领域:
- 纳米技术
- 材料科学
- 生物物理
背景情况:
- 碳纳米粒子 (CNPs) 呈现光发光 (PL),这是对表面相互作用敏感的特性.
- 控制纳米级材料属性对于生物成像等先进应用至关重要.
研究的目的:
- 开发一种可逆切换CNP光发光的方法.
- 研究可切换的CNP在细胞内跟踪和生物成像方面的潜力.
主要方法:
- 有高PL的合成"裸体"负电荷的CNP.
- 通过带正电荷的宏分子进行诱导的PL火.
- 使用阳离子表面活性剂分子恢复PL.
- 使用凝电泳,光谱和体外共聚焦成像验证了切换.
主要成果:
- 通过宏分子相互作用证明了 CNP 的可逆光发转.
- 证实PL是一种依赖充电大分子的表面现象.
- 展示了可切换的CNP在与离子两相互作用时恢复发光的能力.
结论:
- 开发了第一个可切换发光的CNP,用于高效的细胞内追踪.
- 通过宏分子和脱来控制CNP光发光的纳米级方法.
- 提供了与充电大分子相关的CNP光发光的表面来源的证据.
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