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在水中通过疏水编码对的序列选择性结合

Joseph K Awino¹, Roshan W Gunasekara¹, Yan Zhao¹

¹Department of Chemistry, Iowa State University , Ames, Iowa 50011-3111, United States.

Journal of the American Chemical Society

|January 28, 2017

概括

研究人员使用"疏水性编码小穴"开发了纳米粒子受体,以实现特定序列的结合. 这一突破使得特征的精确识别成为可能,

科学领域:

生物技术
材料科学
化学生物学

背景情况:

开发特定序列的结方法是分子识别的长期挑战.
现有的方法往往缺乏精度来区分结构的微妙变化.

研究的目的:

创建一个基于纳米粒子的新系统,用于特定序列的识别.
研究基于疏水相互作用的纳米粒子受体结合的能力.

主要方法:

通过表面核心双重交联的微粒合成水溶性纳米粒子受体.
设计了一系列
含有恐水性编码的小穴
在纳米粒子表面.
根据它们的疏水侧链的位置,数量和性质来识别.

主要成果:

实现高精度的序列特定结.
已经证明能够区分微小的链差异.
在水溶液中获得高达20nM的生物活性寡的结合亲和度.

结论:

开发的纳米粒子受体为特定序列的结提供了通用和有效的方法.
这种方法为分子识别和诊断和治疗中的潜在应用提供了强大的工具.
在
含有恐水性编码的小穴
在设计人工受体方面取得了重大进展.

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