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具有哺乳动物多能干细胞的跨物种化学反应

Jun Wu¹, Aida Platero-Luengo¹, Masahiro Sakurai¹

¹Salk Institute for Biological Studies, 10010 N. Torrey Pines Road, La Jolla, CA 92037, USA.

|January 28, 2017

概括

研究人员在猪和牛胚胎中研究了人类多能干细胞 (hPSC). 中间hPSCs对猪胚胎发育有显著的贡献,提高了异种器官的产生潜力.

科学领域:

发育生物学
干细胞生物学
异种移植研究

背景情况:

跨物种胚胎细胞补充促进异源多能干细胞 (PSC) 衍生物的器官特异性丰富.
克里斯普尔-卡斯9技术可用于研究胚胎发生和疾病模型的多功能胚胎补充平台.
在动物中产生人类器官的潜力需要了解不同哺乳动物物种中人类PSC (hPSC) 的贡献.

研究的目的:

用CRISPR-Cas9为基因编辑器官发育障碍的小鼠建立一个通用的胚胎细胞补充平台.
在类动物 (猪和牛) 中评估各种hPSC类型的化学能力.
确定hPSC对非动物物种未来器官生成中的潜在贡献.

主要方法:

开发CRISPR-Cas9介导的胚胎基因组编辑平台,用于胚胎细胞补充.
系统评估不同类型的hPSC用于猪和牛植入前和植入后的胚胎.
在植入后的猪胚胎中对移植的hPSC产生分化后代的评估.

主要成果:

在经过基因编辑的有机生殖障碍的小鼠的多种组织中丰富了小鼠PSC衍生物.
原始的hPSC在猪和牛的植入前囊中表现出强大的植入.
中间hPSCs表现出更高程度的嵌合性,并在植入后的猪胚胎中产生分化后代,与原始hPSCs相比,它们的贡献有限.

结论:

这项研究成功地建立了一个用于跨物种研究的多功能胚胎细胞补充平台.
介质hPSC显示有望在类动物,特别是猪中促进合体形成.
这些发现提升了人类器官生成的潜力,

关键词:

在CRISPR-Cas9中人类原始多能干细胞人类 - 牛嵌合体胚胎人与猪合体胚胎跨物种的胚胎细胞补充跨物种的木乃伊器官和组织的产生多能干细胞胚胎基因组编辑

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