通过对代谢的全球评估发现细胞类型和疾病相关的酶活性变化
Jun Onagi, Toru Komatsu1, Yuki Ichihashi
1Precursory Research for Embryonic Science and Technology (PRESTO), Japan Science and Technology Agency (JST) , 4-1-8 Honcho Kawaguchi, Saitama 332-0012, Japan.
Journal of the American Chemical Society
|February 14, 2017
概括
这项研究引入了一种新的高通量系统来分析蛋白酶和酶活动. 该平台可以识别与疾病相关的酶,如结直肠瘤中神经溶解的升高,有助于生物标志物和药物点的发现.
科学领域:
- 生物化学和分子生物学
- 酵素学
- 蛋白质组学
背景情况:
- 细胞平衡依赖于酶活动,形成酶体.
- 蛋白酶和酶是与各种疾病相关的关键酶类.
- 了解特定的酶活动是疾病机制和治疗向的关键.
研究的目的:
- 开发和验证用于评估代谢活动的高吞吐量系统.
- 同时识别负责代谢特定基质的酶.
- 发现细胞类型特定和与疾病相关的酶活动及其相应的酶.
主要方法:
- 使用外源性基板同时评估代谢活动.
- 负责定义基质代谢的酶的高通量识别.
- 应用该系统分析细胞类型和疾病特异性的酶活性.
主要成果:
- 开发的系统成功地确定了细胞类型特异性和与疾病相关的外和内酶活动.
- 该平台准确地确定了对观察到的代谢活动负责的特定酶.
- 在人类结直肠瘤组织中检测到内酶神经溶解的活性升高.
结论:
- 开发的酶组学平台是发现细胞类型特异性酶反应的强大工具.
- 该系统可以识别疾病中的潜在生物标志物或药物标的酶功能变化.
- 这种方法通过阐明潜在的酶机制来促进疾病病理的调查.
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