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来自热反应模板的交联球核酸

Resham J Banga¹, Brian Meckes¹, Suguna P Narayan¹

¹International Institute of Nanotechnology, ‡Department of Chemical and Biological Engineering, §Department of Chemistry, and ∥Department of Biomedical Engineering, Northwestern University , 2145 Sheridan Road, Evanston, Illinois 60208, United States.

Journal of the American Chemical Society

|February 17, 2017

概括

研究人员为免疫调节开发了稳定的球状核酸 (SNA) 纳米结构. 这些SNA结构有效地刺激托尔类受体9 (TLR-9),为免疫调节应用提供增强的稳定性和细胞吸收.

科学领域:

生物材料科学
纳米技术
免疫学

背景情况:

球形核酸 (SNA) 纳米结构具有治疗潜力,但在生理环境中往往缺乏稳定性.
非共价的SNA结构容易分裂,限制了它们在生物应用中的有效性.
开发稳定且易于内部化的SNA构造对于推进它们在医学中的应用至关重要.

研究的目的:

开发一种新型的一合成方法,用于稳定的小球球核酸 (SNA) 纳米结构.
使用Pluronic F127作为SNA制造的热响应模板.
为免疫调节应用创建具有增强稳定性和细胞内化的SNA结构.

主要方法:

一种单合成,涉及将DNA两基 (CpG动机) 插入到Pluronic F127基中.
化学交叉链接的交叉链接 DNA 两种类型形成一个稳定的纳米结构.
移除非交叉连接的组件以获得最终的状SNA结构.

主要成果:

通过密集的核酸外成功合成了小细胞SNA纳米结构.
在生理介质中证明了交叉结合的小神经元的稳定性.
观察到SNA结构的快速细胞内化,表明有效的传递.

结论:

开发出来的细胞SNA纳米结构为免疫调节提供了稳定有效的平台.
这些结构是强大的托尔类受体9 (TLR-9) 激动剂,突出显示了它们的免疫调节潜力.
合成解决了非共价SNA结构的局限性,为新的治疗策略铺平了道路.

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