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相关概念视频

Types of RNA01:20

Types of RNA

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Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in regulating gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA Performs Diverse...
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Types of RNA01:23

Types of RNA

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Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
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Nucleic Acids02:43

Nucleic Acids

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Nucleic acids are the most important macromolecules for the continuity of life. They carry the cell's genetic blueprint and carry instructions for its functioning.
DNA and RNA
The two main types of nucleic acids are deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). DNA is the genetic material in all living organisms, ranging from single-celled bacteria to multicellular mammals. It is in the nucleus of eukaryotes and in the organelles, chloroplasts, and mitochondria. In prokaryotes,...
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Nucleic acids02:43

Nucleic acids

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Nucleic acids are the most important macromolecules for the continuity of life. They carry the cell's genetic blueprint and carry instructions for its functioning.
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RNA Polymerase II Accessory Proteins02:36

RNA Polymerase II Accessory Proteins

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Proteins that regulate transcription can do so either via direct contact with RNA Polymerase or through indirect interactions facilitated by adaptors, mediators, histone-modifying proteins, and nucleosome remodelers. Direct interactions to activate transcription is seen in bacteria as well as in some eukaryotic genes. In these cases, upstream activation sequences are adjacent to the promoters, and the activator proteins interact directly with the transcriptional machinery. For example, in...
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RNA Polymerase II Accessory Proteins02:36

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Isolation of Cognate RNA-protein Complexes from Cells Using Oligonucleotide-directed Elution
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在同一基因内连接蛋白质和RNA功能

Anthony Szempruch1, Mitchell Guttman1

  • 1Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, California 91125, USA.

Cell
|February 25, 2017
PubMed
概括
此摘要是机器生成的。

紫外线会触发DNA损伤反应, 全球减少转录. 一种新发现的蛋白质及其相关的非编码RNA帮助细胞从这种转录抑制状态中恢复.

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相关实验视频

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科学领域:

  • 分子生物学
  • 遗传学
  • 细胞对DNA损伤的反应

背景情况:

  • 紫外线 (UV) 辐射暴露会引起显著的细胞应激.
  • 紫外线暴露会激活整个细胞的DNA损伤反应 (DDR).
  • DDR的一个关键特征是细胞转录的总体减少.

研究的目的:

  • 确定细胞对紫外线引起的DNA损伤的关键分子参与者.
  • 研究特定蛋白质和非编码RNA在DDR期间调节转录中的作用.
  • 了解促进紫外线诱导的转录抑制的机制.

主要方法:

  • 使用分子生物学技术识别参与紫外线反应的蛋白质.
  • 使用RNA测序和分析来描述非编码RNA的产生.
  • 研究了已识别的蛋白质和非编码RNA在细胞恢复中的功能作用.

主要成果:

  • 发现了一种特定的蛋白质, 关键是对紫外线的DNA损伤反应.
  • 发现了一种通过替代RNA处理生成的新型非编码RNA.
  • 证明这种非编码RNA可促进紫外线诱导的转录抑制的恢复.

结论:

  • 一种特定的蛋白质和一种新的非编码RNA是细胞对紫外线诱导的DNA损伤反应的关键调节者.
  • 非编码RNA有助于在紫外线暴露后恢复转录.
  • 这些发现为DNA修复和恢复的细胞机制提供了洞察力.