古代配体融合蛋白HAP2是一种真核生物II类融合蛋白
Juliette Fédry1, Yanjie Liu2, Gérard Péhau-Arnaudet3
1Unité de Virologie Structurale, Institut Pasteur, 25-28 Rue du Docteur Roux, 75724 Paris, France; CNRS UMR 3569, 25-28 Rue du Docteur Roux, 75724 Paris, France; Université Paris Descartes Sorbonne Paris Cité, Institut Pasteur, Rue du Docteur Roux, 75015 Paris, France; Institute of Virology, Hannover Medical School, 30625 Hannover, Germany.
Cell
|February 25, 2017
概括
在许多真核生物中,HAP2蛋白对子细胞的融合至关重要,其作用与病毒融合蛋白相似. 这一发现提供了一种阻止疟疾寄生虫传播的潜在策略.
科学领域:
- 细胞生物学
- 分子和结构生物学
- 进化生物学
背景情况:
- 性繁殖涉及雌同体的融合,这是真核生物生命的关键过程.
- HAP2-GCS1蛋白是一种保存的,精子特异性的跨膜蛋白,涉及到膜融合.
- 在多种真核生物种中发现HAP2的存在表明了已保存的配体融合机制.
研究的目的:
- 阐明HAP2蛋白在雌同体融合中的功能和机制.
- 研究HAP2的进化起源和结构同质性.
- 探索HAP2作为阻止病原体传播的目标的潜力.
主要方法:
- 生物信息分析以确定HAP2同源性.
- 用于研究HAP2功能的生物化学测试.
- 针对位点的突变发生, 探测融合环区域.
- 用X射线结晶学进行结构测定.
- 抗体介导的细胞融合抑制.
主要成果:
- 来自Chlamydomonas reinhardtii的HAP2与II类病毒膜融合蛋白具有同质性.
- HAP2融合循环或抗体阻塞的突变抑制了雌激素融合.
- 结构和功能数据证实了HAP2作为配体融合因子的作用.
- HAP2的作用机制类似于病毒融合蛋白.
结论:
- 在真核生物中,HAP2是保存的配体融合基因.
- HAP2通过类似于病毒膜融合的机制起作用.
- 针对HAP2提供了一个潜在的策略来抑制Plasmodium的传播.
- 病毒细胞基因交换可能影响了真核细胞融合机制的进化.
相关概念视频
Tagging and Fusion Proteins
8.6K
Proteins are involved in several cellular processes and biochemical reactions. Analyzing a specific protein of interest requires it to be isolated from the other proteins in the cell. This is achieved by overexpressing the specific gene in a suitable host to produce large quantities of the target protein. A tag or label is recombined with the gene to produce a fusion protein containing the target protein and the tag. The tags on these fusion proteins can then be used for easy detection and...
8.6K
Protein Complexes with Interchangeable Parts
3.0K
Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order...
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order...
3.0K
Hybridoma Technology
18.0K
Hybridoma technology is used for the large-scale production of monoclonal antibodies. Monoclonal antibodies bind to only a single antigenic determinant or epitope. Such antibodies are used in research, diagnostics, and disease therapy. The hybridoma technology established in 1975 by Georges Köhler and Cesar Milstein was awarded the Nobel Prize in Medicine in 1984 for revolutionizing research and therapy.
Hybridoma Selection
Commonly used fusion techniques — electroporation,...
Hybridoma Selection
Commonly used fusion techniques — electroporation,...
18.0K
Exon Recombination
4.2K
The evolution of new genes is critical for speciation. Exon recombination, also known as exon shuffling or domain shuffling, is an important means of new gene formation. It is observed across vertebrates, invertebrates, and in some plants such as potatoes and sunflowers. During exon recombination, exons from the same or different genes recombine and produce new exon-intron combinations, which might evolve into new genes.
Exon shuffling follows “splice frame rules.” Each exon...
Exon shuffling follows “splice frame rules.” Each exon...
4.2K


