人类长非编码RNA与精确的5'端的图谱
Chung-Chau Hon1, Jordan A Ramilowski1,2, Jayson Harshbarger1,2
1RIKEN Center for Life Science Technologies (Division of Genomic Technologies), 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama, 230-0045 Japan.
Nature
|February 28, 2017
概括
这项研究提供了一个全面的人类长非编码RNA (lncRNA) 地图,识别了数千种潜在的功能lncRNA及其在基因调节和疾病中的作用.
科学领域:
- 基因组学
- 转录组学
- 表观遗传学
背景情况:
- 长非编码RNAs (lncRNAs) 是一个多样化的分子类别,其功能在很大程度上是未知的.
- 现有的转录库是零碎的,阻碍了对 lncRNA 的全面理解.
研究的目的:
- 创建一个人体 lncRNA 基因的综合图谱.
- 根据基因组和表观基因组特征对 lncRNA 进行分类.
- 研究 lncRNAs 在基因调节和疾病中的功能作用.
主要方法:
- 将FANTOM5基因表达分析 (CAGE) 数据与多个转录收集集成.
- 对 lncRNA 的基因组和表观基因组分类.
- 在不同的人类细胞类型和组织中分析 lncRNA表达特征.
- 遗传,表达和保护数据的整合.
主要成果:
- 产生了27,919个人类lncRNA基因的高可靠性地图.
- 发现大多数基因间 lncRNA 来自增强剂.
- 与特征相关的SNP重叠的lncRNA显示了与特征相关的细胞类型特异性表达.
- 与目标mRNA共同表达的ncRNA重叠eQTL相关SNP,这表明它们具有调节作用.
- 发现了19175个潜在的功能性 lncRNA.
结论:
- 这项研究为IncRNA研究提供了宝贵的资源.
- lncRNAs在转录调节中起着重要作用,并与各种疾病有关.
- 这本地图书有助于进一步描述 lncRNA 的功能.
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