体WO基因重复并增强沃尔巴基亚诱导的细胞质不相容性
Daniel P LePage1, Jason A Metcalf1, Sarah R Bordenstein1
1Department of Biological Sciences, Vanderbilt University, Nashville, Tennessee 37235, USA.
Nature
|February 28, 2017
概括
研究人员确定了两个基因, cifA和 cifB,负责沃尔巴基亚诱导的细胞质不相容性. 这一发现有助于我们更好地理解生殖操纵和沃尔巴基亚.
科学领域:
- 微生物学
- 遗传学
- 进化生物学
背景情况:
- 沃尔巴基亚是细胞内细菌感染无脊椎动物,操纵繁殖以增加它们的流行.
- 细胞质不相容 (CI) 是一种常见的沃尔巴基亚诱导的生殖操纵,导致特定交叉的胚胎死亡.
- 尽管它对共生体诱导的物种化和害虫控制具有重要意义,但CI的遗传基础仍然难以捉摸.
研究的目的:
- 确定负责沃尔巴基亚介导细胞质不相容的细菌基因.
- 使用转基因方法对已识别的基因进行功能验证.
主要方法:
- 传播WO基因的比较基因组学.
- 在Drosophila melanogaster中,候选基因 (cifA和 cifB) 的转基因表达.
- 在涉及转基因的杂交中评估胚胎死亡率.
主要成果:
- 鉴定了两个共同分离的基因, cifA和 cifB,来自预兆WO的真核关联模块.
- 在未感染的雄性Drosophila中, cifA和 cifB的双重表达得到了回归和增强CI.
- 观察到的胚胎死亡率模仿了野生类型的CI,并被Wolbachia感染的胚胎所拯救.
结论:
- 基因 cifA 和 cifB 是导致 Wolbachia 中细胞质不相容的主要因素.
- 这一发现为Wolbachia繁殖操纵的基因研究开辟了新的途径.
- 这些发现支持使用沃尔巴基亚来控制传递登革热和寨卡病毒的树木病毒.
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