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In Vitro Biochemical Assays using Biotin Labels to Study Protein-Nucleic Acid Interactions
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通过含有核基氨基酸的蛋白质表现出的i-图案DNA基质的增强结合亲和力

Xiaoguang Bai1, Poulami Talukder1, Sasha M Daskalova1

  • 1Biodesign Center for BioEnergetics, and School of Molecular Sciences, Arizona State University , Tempe, Arizona 85287, United States.

Journal of the American Chemical Society
|March 7, 2017
PubMed
概括

研究人员修改了一种蛋白质

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Binding sites linkages can regulate a protein's function.  For example, enzyme activity is often regulated through a feedback mechanism where the end product of the biochemical process serves as an inhibitor.
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Conserved Binding Sites

Many proteins’ biological role depends on their interactions with their ligands, small molecules that bind to specific locations on the protein known as ligand-binding sites. Ligand-binding sites are often conserved among homologous proteins as these sites are critical for protein function.
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Single-Strand DNA Binding Proteins

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Ligand Binding Sites

Proteins are dynamic macromolecules that carry out a wide variety of essential processes; however, the activities of most proteins depend on their interactions with other molecules or ions, known as ligands.
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