蛋白质中电子增益和损失中心的结构和运动
G D Jones1, J S Lea, M C Symons
1Department of Chemistry, University of Leicester, UK.
Nature
|December 24, 1987
概括
电子自旋共振光谱显示,骨干胺基基是由辐射诱导的蛋白质中电子损失形成的主要物种. 这种技术对于理解生物系统中的辐射损伤机制至关重要.
科学领域:
- 生物物理学的生物物理.
- 辐射生物学 辐射生物学
- 频谱学是一种光谱学.
背景情况:
- 蛋白质辐射损伤是复杂的,最终产品难以识别.
- 含有不配对电子的自由基是辐射损伤的关键中间体.
- 电子自旋共振 (ESR) 光谱对自由基很敏感.
研究的目的:
- 为了识别蛋白质中辐射损伤形成的初级和二级基因物种.
- 阐明辐射诱导蛋白质损伤的基本过程.
- 为了研究电子的移动性和蛋白质中的捕获.
主要方法:
- 使用了电子自旋共振 (ESR) 光谱.
- 在低温下对一系列蛋白质进行辐射.
- 分析了对14N核的超细合以进行基因鉴定.
主要成果:
- 确定了骨干胺基基, -N.(CO) - ,作为蛋白质中电子损失形成的主要物种.
- 观察到在低温下有效地捕获这些胺基.
- 证明了蛋白质中的电子流动性,电子被像DNA这样的电友中心捕获.
结论:
- 骨干胺基基是蛋白质中辐射诱导的电子损失的重要产物.
- ESR光谱是一种强大的工具,用于表征生物分子中辐射诱导的激素.
- 电子流动性在生物系统中辐射损伤的分布和结果中起着至关重要的作用.
相关概念视频
Mechanical Protein Functions
Proteins perform many mechanical functions in a cell. These proteins can be classified into two general categories- proteins that generate mechanical forces and proteins that are subjected to mechanical forces. Proteins providing mechanical support to the structure of the cell, such as keratin, are subjected to mechanical force, whereas proteins involved in cell movement and transport of molecules across cell membranes, such as an ion pump, are examples of generating mechanical force.
Structural Protein Function
Structural proteins are a category of proteins responsible for functions ranging from cell shape and movement to providing support to major structures such as bones, cartilage, hair, and muscles. This group includes proteins such as collagen, actin, myosin, and keratin.
Collagen, the most abundant protein in mammals, is found throughout the body. In connective tissue, such as skin, ligaments, and tendons, it provides tensile strength and elasticity. In bones and teeth, it mineralizes to form...
Collagen, the most abundant protein in mammals, is found throughout the body. In connective tissue, such as skin, ligaments, and tendons, it provides tensile strength and elasticity. In bones and teeth, it mineralizes to form...
Electronic Structure of Atoms
An atom comprises protons and neutrons, which are contained inside the dense, central core called the nucleus, with electrons present around the nucleus. Taking into account the wave–particle duality of electrons and the uncertainty in position around the nucleus, quantum mechanics provides a more accurate model for the atomic structure. It describes atomic orbitals as the regions around the nucleus where electrons of discrete energy exist, characterized by four quantum numbers: n, l, ml, and...
ATP Synthase: Structure
ATP synthase or ATPase is among the most conserved proteins found in bacteria, mammals, and plants. This enzyme can catalyze a forward reaction in response to the electrochemical gradient, producing ATP from ADP and inorganic phosphate. ATP synthase can also work in a reverse direction by hydrolyzing ATP and generating an electrochemical gradient. Different forms of ATP synthases have evolved special features to meet the specific demands of the cell. Based on their specific feature, ATP...
Mechanical Protein Function
Proteins perform many mechanical functions in a cell. These proteins can be classified into two general categories- proteins that generate mechanical forces and proteins that are subjected to mechanical forces. Proteins providing mechanical support to the structure of the cell, such as keratin, are subjected to mechanical force, whereas proteins involved in cell movement and transport of molecules across cell membranes, such as an ion pump, are examples of generating mechanical force.
Structural Protein Function
Structural proteins are a category of proteins responsible for functions ranging from cell shape and movement to providing support to major structures such as bones, cartilage, hair, and muscles. This group includes proteins such as collagen, actin, myosin, and keratin.
Collagen, the most abundant protein in mammals, is found throughout the body. In connective tissue, such as skin, ligaments, and tendons, it provides tensile strength and elasticity. In bones and teeth, it mineralizes to form...
Collagen, the most abundant protein in mammals, is found throughout the body. In connective tissue, such as skin, ligaments, and tendons, it provides tensile strength and elasticity. In bones and teeth, it mineralizes to form...


