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Published on: July 27, 2012
电子从蛋白质转移到DNA在辐射的染色中
P M Cullis1, G D Jones, M C Symons
1Department of Chemistry, University of Leicester, UK.
Nature
|December 24, 1987
概括
通过研究DNA-蛋白质复合体中的电子转移,可以更好地理解辐射对DNA的损伤. 当DNA与基因素复合时,DNA中的电子增益中心会增加,这表明真核生物中DNA损伤的新途径.
科学领域:
- 生物物理学的生物物理.
- 分子生物学分子生物学
- 辐射化学 辐射化学
背景情况:
- 辐射对DNA的直接损伤涉及胺上的电子增益中心和关氨酸上的电子损失中心.
- 这些基因中心是DNA链断裂的前体,可能与体内损伤途径有关.
研究的目的:
- 为了研究DNA-蛋白质复合对辐射诱导的基因中心形成的影响.
- 为了确定基质子和DNA之间的电子转移是否会影响DNA损伤产量.
主要方法:
- 电子自旋共振 (ESR) 光谱被用来检测和量化DNA和DNA-蛋白质复合体中的基因中心.
- 研究对干燥和水合冷DNA系统进行了研究,以及与基因素蛋白复合的DNA.
主要成果:
- 仅仅对DNA的辐射就产生了相同的电子增益中心 (胺基离子,T.-) 和电子损失中心 (瓜基离子,G+.) 的产量.
- 在DNA - 基因组复合体中,从基因组到DNA的轻易电子转移显著增加了T.-的产量.
- 在蛋白质中产生的电子损失中心 ("洞") 被困住了,并没有增加DNA中的G +产量.
结论:
- DNA-蛋白质复合改变了辐射诱导的基因形成,有利于DNA中的电子增益.
- 从基质子转移到DNA的电子转移是真核细胞核中DNA损伤途径的一个重要因素.
- 这些发现为生物体内DNA损伤的机制提供了见解.
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