在大肠杆菌中使用工程化生物脂肪酸生物合成途径产生奇碳二碳酸
Robert W Haushalter1,2, Ryan M Phelan1,2, Kristina M Hoh1,2
1Joint BioEnergy Institute, U.S. Department of Energy , 5885 Hollis Street, Emeryville, California 94608, United States.
Journal of the American Chemical Society
|March 15, 2017
概括
研究人员开发了一种新的基于生物的方法, 这种新的工程大肠杆菌途径为生产这些有价值的工业化学品提供了可持续的替代方案.
科学领域:
- 生物技术和代谢工程
- 工业化学和生物加工
背景情况:
- 二碳酸是重要的商品化学物质,具有广泛的工业应用,包括塑料,聚和药品.
- 目前的生产主要依赖于以石油为基础的方法,这引发了环境问题,并推动了寻找可持续的替代品.
- 现有的生物基路径主要产生偶碳二碳酸,限制了需要奇碳变体的应用选择.
研究的目的:
- 设计一种新的生物合成途径, 从葡萄糖中直接生产二碳酸.
- 解决现有微生物系统的局限性,这些微生物系统主要生产偶碳二碳酸.
- 为开发能够生产有价值的奇偶碳二酸的工业品种奠定基础.
主要方法:
- 通过结合生物素和脂肪酸合成途径的酶, 设计了大肠杆菌的新代谢途径.
- 使用葡萄糖作为奇碳二碳酸生物合成的直接原料.
- 专注于开发一种能够产生特定奇异碳链长度二酸的微生物系统.
主要成果:
- 在大肠杆菌中成功证明了从葡萄糖中直接生物合成奇偶碳二碳酸的新途径.
- 克服了在之前报告的系统中观察到的仅生产均碳物种的限制.
- 通过微生物发酵产生特定的奇异碳二酸的概念证明.
结论:
- 一个新的生物基途径已经建立,直接从葡萄糖中产生奇数碳二碳酸.
- 这种在大肠杆菌中设计的途径与现有的仅限于均碳产品的方法相比,是一个显著的进步.
- 进一步优化有望开发工业微生物菌株以可持续生产有价值的奇偶碳二酸.
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