在哺乳动物细胞中,GAL4激活基因表达
1Department of Biochemistry and Molecular Biology, Harvard University, Cambridge, Massachusetts 02138.
Cell
|January 29, 1988
概括
酵母转录因子GAL4激活了哺乳动物细胞中的基因表达. 它的激活区域和DNA结合能力对于这种跨物种功能至关重要.
科学领域:
- 分子生物学分子生物学
- 基因规则 基因规则
- 酵母遗传学 酵母遗传学
背景情况:
- 在酵母中,GAL4是一种特征很好的转录激活剂.
- 小鼠乳腺瘤病毒 (MMTV) 促销者是基因表达研究中常见的记者.
- 了解跨物种转录因子的功能,可以了解保存的调节机制.
研究的目的:
- 调查酵母转录因子GAL4是否可以激活哺乳动物细胞中的转录.
- 确定GAL4在哺乳动物系统中激活所需的功能域.
- 检查GAL4和葡萄糖皮质体受体对MMTV促进体的协同作用.
主要方法:
- 用含有 GAL4 DNA 结合序列和记者基因的等离子体感染哺乳动物细胞.
- 对记者基因表达的分析.
- 使用GAL4删除突变体和DNA结合缺陷突变体.
- 与葡萄糖皮质体受体表达载体共传染.
主要成果:
- 在哺乳动物细胞中,GAL4会激活MMTV促进体.
- 激活需要GAL4结合序列在促进体的上游.
- 特定的GAL4激活区域在哺乳动物细胞中的功能得到保护.
- 一种与DNA结合但不能在酵母中激活的GAL4衍生物也无法在哺乳动物细胞中激活.
- GAL4和葡萄皮质皮质体受体表现出MMTV促进体的协同激活.
结论:
- GAL4转录因子可以在哺乳动物细胞中起作用,这表明了保存的调节原则.
- 在GAL4内部的特定域对于其跨物种转录激活至关重要.
- GAL4和葡萄糖皮质体受体可以合作增强基因表达.
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