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相关概念视频

Replication in Eukaryotes02:31

Replication in Eukaryotes

Overview
The DNA Replication Fork01:02

The DNA Replication Fork

An organism’s genome needs to be duplicated in an efficient and error-free manner for its growth and survival. The replication fork is a Y-shaped active region where two strands of DNA are separated and replicated continuously. The coupling of DNA unzipping and complementary strand synthesis is a characteristic feature of a replication fork.   Organisms with small circular DNA, such as E. coli, often have a single origin of replication; therefore, they have only two replication forks, one in...
Restarting Stalled Replication Forks02:37

Restarting Stalled Replication Forks

DNA replication is initiated at sites containing predefined DNA sequences known as origins of replication. DNA is unwound at these sites by the minichromosome maintenance (MCM) helicase and other factors such as Cdc45 and the associated GINS complex.The unwound single strands are protected by replication protein A (RPA) until DNA polymerase starts synthesizing DNA at the 5’ end of the strand in the same direction as the replication fork. To prevent the replication fork from falling apart, a...
The DNA Replication Fork01:02

The DNA Replication Fork

An organism’s genome needs to be duplicated in an efficient and error-free manner for its growth and survival. The replication fork is a Y-shaped active region where two strands of DNA are separated and replicated continuously. The coupling of DNA unzipping and complementary strand synthesis is a characteristic feature of a replication fork.   Organisms with small circular DNA, such as E. coli, often have a single origin of replication; therefore, they have only two replication forks, one in...
Replication in Eukaryotes01:29

Replication in Eukaryotes

In eukaryotic cells, DNA replication is highly conserved and tightly regulated. Multiple linear chromosomes must be duplicated with high fidelity before cell division, so there are many proteins that fulfill specialized roles in the replication process. Replication occurs in three phases: initiation, elongation, and termination, and ends with two complete sets of chromosomes in the nucleus.
Many Proteins Orchestrate Replication at the Origin
Eukaryotic replication follows many of the same...
Restarting Stalled Replication Forks02:37

Restarting Stalled Replication Forks

DNA replication is initiated at sites containing predefined DNA sequences known as origins of replication. DNA is unwound at these sites by the minichromosome maintenance (MCM) helicase and other factors such as Cdc45 and the associated GINS complex.The unwound single strands are protected by replication protein A (RPA) until DNA polymerase starts synthesizing DNA at the 5’ end of the strand in the same direction as the replication fork. To prevent the replication fork from falling apart, a...

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相关实验视频

Updated: Jul 17, 2026

Direct Observation of Enzymes Replicating DNA Using a Single-molecule DNA Stretching Assay
17:03

Direct Observation of Enzymes Replicating DNA Using a Single-molecule DNA Stretching Assay

Published on: March 23, 2010

启动蛋白诱导了lambda起源的螺旋不稳定:DNA复制中的一个预备步骤.

M Schnos1, K Zahn, R B Inman

  • 1Institute for Molecular Virology, University of Wisconsin-Madison 53706.

Cell
|February 12, 1988
PubMed
概括

兰巴菌体启动蛋白O与原始序列结合,导致对复制启动至关重要的DNA结构变化. 这一过程依赖于DNA超螺旋张力,是双向复制的第一步.

科学领域:

  • 分子生物学分子生物学
  • 遗传学 遗传学 是一个
  • 生物化学 生物化学

背景情况:

  • 兰巴菌体启动蛋白O与复制的兰巴起源 (ori) 结合.
  • 了解DNA复制的初始分子事件对于理解病毒传播和基因组稳定性至关重要.

研究的目的:

  • 为了研究启动蛋白O.的结合时,兰巴达起源序列的结构变化.
  • 确定DNA超螺旋张力在O-ori相互作用中的作用及其对复制启动的影响.

主要方法:

  • 核酶敏感性测定用于检测DNA中的结构修饰.
  • 实验涉及野生类型和突变原始序列,以评估观察到的变化的功能相关性.

主要成果:

  • 蛋白O的结合会在ori序列的特定AT丰富区域引起显著的结构变化.
  • 这种DNA修饰是独立于ATP水解的,并且通过非复制原始突变减少.
  • 结构变化严重依赖于DNA中的超螺旋张力,并表现出链分离的特征.

结论:

  • 兰巴菌体启动蛋白O与原点序列之间的相互作用会诱导一种取决于张力的DNA结构修饰.
  • 这种修改被提出为一个基本的预事件,代表了在lambda菌体中启动双向DNA复制的最初步骤.

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Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method
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Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method

Published on: May 2, 2025

Single-Molecule Real-Time Visualization of DNA Unwinding by CMG Helicase
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Single-Molecule Real-Time Visualization of DNA Unwinding by CMG Helicase

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相关实验视频

Last Updated: Jul 17, 2026

Direct Observation of Enzymes Replicating DNA Using a Single-molecule DNA Stretching Assay
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Direct Observation of Enzymes Replicating DNA Using a Single-molecule DNA Stretching Assay

Published on: March 23, 2010

Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method
08:53

Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method

Published on: May 2, 2025

Single-Molecule Real-Time Visualization of DNA Unwinding by CMG Helicase
07:37

Single-Molecule Real-Time Visualization of DNA Unwinding by CMG Helicase

Published on: September 27, 2024