在真核细胞中对DNA复制进行Cis作用的负控制
1Division of Basic Sciences, Fred Hutchinson Cancer Center, Seattle, Washington 98104.
Cell
|February 12, 1988
概括
嵌合体SV40-BPV插曲的复制每细胞周期发生一次,由cis作用的负机制控制. 这确保了通过防止在同一细胞周期内重复复制的稳定插曲维护.
科学领域:
- 分子生物学分子生物学
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 病毒学 病毒学
背景情况:
- 插件复制体需要精确的细胞周期控制来保持稳定的副本数量.
- 了解控制DNA复制启动和终止的机制对于基因组稳定至关重要.
研究的目的:
- 为了研究一个仿真猿类病毒40-牛乳头瘤病毒 (SV40-BPV) 的细胞周期依赖的复制控制.
- 为了确定监管机制,确保每细胞循环复制一次的突发性DNA.
主要方法:
- 使用了稳定的子细胞系,其中含有仿真SV40-BPV插曲性复制体.
- 分析了细胞周期中的复制频率和情节复制数维护.
- 研究了T抗原启动因子在复制控制中的作用.
主要成果:
- 这种SV40-BPV插曲性复制体在每个细胞周期中完全复制一次.
- 情节拷贝数保持稳定,即使有过多的T抗原.
- 确定了一个cis作用的负控制机制,防止重复发作的重复复制.
结论:
- 这一情节的复制控制是由一种新的 cis 作用的负面机制介导的,而不是通过限制积极因素.
- 在SV40 T抗原形成一个稳定的,潜在的遗传关联与病变模板后复制.
相关概念视频
01:23Negative Regulator Molecules
Positive regulators allow a cell to advance through cell cycle checkpoints. Negative regulators have an equally important role as they terminate a cell’s progression through the cell cycle—or pause it—until the cell meets specific criteria.
02:36DNA Damage can Stall the Cell Cycle
In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
02:46Genome Copying Errors
DNA replication is a well-evolved process that copies millions of base pairs with high fidelity during each cell division. Occasionally a wrong base or a long stretch of wrong bases may get added to the daughter strands. If the errors are left unchecked, cells might accumulate several mutations that might endanger their survival. Therefore, the copying errors are checked and repaired at three levels.
02:38S-Cdk Initiates DNA Replication
The cell cycle is a series of events leading to DNA duplication followed by the division of cell content to form two daughter cells. The cell cycle progresses in four stages—the cell increases in size (gap 1 or G1-phase), duplicates its DNA (synthesis or S-phase), prepares to divide (gap 2 or G2-phase), and divides (mitosis or M-phase).
Two states at the origin of replication
In eukaryotes, the initiation of replication occurs at many sites on the chromosomes, called the origins of replication.
Two states at the origin of replication
In eukaryotes, the initiation of replication occurs at many sites on the chromosomes, called the origins of replication.
02:36DNA Damage Can Stall the Cell Cycle
In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
02:38S-Cdk Initiates DNA Replication
The cell cycle is a series of events leading to DNA duplication followed by the division of cell content to form two daughter cells. The cell cycle progresses in four stages—the cell increases in size (gap 1 or G1-phase), duplicates its DNA (synthesis or S-phase), prepares to divide (gap 2 or G2-phase), and divides (mitosis or M-phase).
Two states at the origin of replication
In eukaryotes, the initiation of replication occurs at many sites on the chromosomes, called the origins of replication.
Two states at the origin of replication
In eukaryotes, the initiation of replication occurs at many sites on the chromosomes, called the origins of replication.