相关实验视频
Updated: May 11, 2026

09:40
Analysis of the c-KIT Ligand Promoter Using Chromatin Immunoprecipitation
Published on: June 27, 2017
一个核因子1结合部位通过转化增长因子-β来调节I型原蛋白促进体的转录激活
P Rossi1, G Karsenty, A B Roberts
1Laboratory of Molecular Biology, National Cancer Institute, Bethesda, Maryland 20892.
Cell
|February 12, 1988
概括
转化生长因子-β (TGF-β) 激活了原蛋白基因转录. 这个过程需要一个特定的核因子1 (NF1) 结合位点,这表明它在TGF-β中起着至关重要的作用.
科学领域:
- 分子生物学分子生物学
- 细胞生物学 细胞生物学
- 生物化学 生物化学
背景情况:
- 已知转化生长因子-β (TGF-β) 调节纤维细胞中细胞外基质蛋白的产生.
- 了解TGF-β作用背后的转录机制对于研究组织重塑和纤维化至关重要.
研究的目的:
- 研究特定DNA元素在调解TGF-β诱导的小鼠α2 (I) 原基因的转录激活中的作用.
- 为了确定参与TGF-β信号通路的转录因子.
主要方法:
- 在NIH 3T3和骨肉瘤细胞中利用了DNA转染试验.
- 在小鼠α2 (I) 原促进体上进行了删除和位点定向突变发生的分析.
- 通过测量记者基因表达来评估促进体活性.
- 使用异质促进剂 (SV40早期促进剂) 测试了已识别的调节元件的功能.
主要成果:
- TGF-β显著增加了小鼠α2 (I) 原促进体的活性 (5至10倍).
- 在-350和-300之间的关键促进子区域,含有核因子1 (NF1) 结合部位,对于TGF-β刺激至关重要.
- 突变NF1结合部位取消了TGF-β诱导的激活.
- 一个NF1结合部位赋予了TGF-β诱导性给异质促进体,证实了其作为调解者的作用.
结论:
- 一个核因子1 (NF1) 的结合部位通过TGF-β.介导鼠标α2 (I) 原促进体的转录激活.
- 这一发现阐明了TGF-β调节细胞外基因表达的关键分子机制.
相关概念视频
Eukaryotic Transcription Activators
Transcription activators are proteins that promote the transcription of genes from DNA to RNA. In most cases, these proteins contain two separate domains ‒ a domain that binds to DNA and a domain for activating transcription; however, in some cases, a single domain is responsible for both binding and activation of transcription, as seen in the glucocorticoid receptor and MyoD.
The binding domains are capable of recognizing and interacting with regulatory sequences on the DNA. These domains are...
The binding domains are capable of recognizing and interacting with regulatory sequences on the DNA. These domains are...
General Transcription Factors
Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
Transcription Elongation Factors
Transcription elongation is a dynamic process that alters depending upon the sequence heterogeneity of the DNA being transcribed. Hence, it is not surprising that the elongation complex's composition also varies along the way while transcribing a gene.
The transcription elongation is regulated via pausing of RNA polymerase on several occasions during transcription. In bacteria, these halts are necessary because the transcription of DNA into mRNA is coupled to the translation of that mRNA into a...
The transcription elongation is regulated via pausing of RNA polymerase on several occasions during transcription. In bacteria, these halts are necessary because the transcription of DNA into mRNA is coupled to the translation of that mRNA into a...
TGF - β Signaling Pathway
The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors are of three kinds RI, RII, and RIII. The RI...
Fibril-associated Collagen
Fibril-associated collagens are a type of collagens present in the extracellular matrix with interrupted triple helices or FACIT (Fibril-associated collagens interrupted triple-helices). FACIT help connect and attach the collagen fibrils with each other as well as with other proteins of the extracellular matrix.
For example, the type II collagen fibrils in cartilage have covalently bound type IX fibril-associated collagens at regular intervals. Other types of fibril-associated collagens are...
For example, the type II collagen fibrils in cartilage have covalently bound type IX fibril-associated collagens at regular intervals. Other types of fibril-associated collagens are...
Type IV Collagen of Basal Lamina
Type IV collagen is a 400 nm long, network-forming collagen that acts as a barrier between the epithelial and endothelial cells. Type IV collagen forms the backbone of the basement membrane by scaffolding with laminin, entactin, proteoglycans, and fibronectin. Apart from rendering structural support to the basement membrane, it also helps entail signaling potentials necessary for both pathological and physiological functions.
A type IV collagen molecule has six alpha chains which can exist in...
A type IV collagen molecule has six alpha chains which can exist in...

