在细胞组件和HSV结构多之间形成的复合物与病毒直接早期基因调节DNA序列结合
C M Preston1, M C Frame, M E Campbell
1Medical Research Council Virology Unit, Institute of Virology, Glasgow, Scotland.
Cell
|February 12, 1988
概括
疹简单病毒 (HSV) 蛋白Vmw65与TAATGARAT序列结合,形成病毒诱导的蛋白质-DNA复合体 (IEC). 这种复杂的,涉及Vmw65和细胞因子,有助于调节基因转录.
科学领域:
- 分子生物学分子生物学
- 病毒学 病毒学
- 基因规则 基因规则
背景情况:
- 疹简单病毒 (HSV) 的直接早期基因转录受病毒因素的调节.
- 已知病毒蛋白Vmw65可以刺激这种转录过程.
- 特定的DNA序列对于这种相互作用至关重要.
研究的目的:
- 调查Vmw65刺激HSV立即早期基因转录的机制.
- 为了识别和描述在这个过程中形成的蛋白质-DNA复合体.
- 了解Vmw65和细胞因子在特定序列DNA结合中的作用.
主要方法:
- 从感染HSV的细胞中化核提取物与TAATGARATDNA片段.
- 将含有Vmw65的维龙提取物添加到未感染细胞的核提取物中.
- 检测和描述病毒诱导的蛋白质-DNA复合体 (IEC).
主要成果:
- 在将核提取物与TAATGARAT DNA 片段化后检测到一种新型病毒诱导的蛋白质-DNA 复合体 (IEC).
- 当含有Vmw65的virion提取物被添加到未感染细胞的核提取物中时,也观察到IEC的形成.
- Vmw65被确定为IEC的一个组件,该组件特别与TAATGARAT序列结合.
结论:
- Vmw65与细胞因子结合,形成了一个特定序列的DNA结合复合体 (IEC).
- 这种复合体在 HSV 直接早期基因的转录调节中起着至关重要的作用.
- 鉴定的系统可以作为研究可诱导细胞基因调节的模型.
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