血小板衍生生长因子在中枢神经系统正常质生成中的作用
W D Richardson1, N Pringle, M J Mosley
1Department of Biology, University College, London, England.
Cell
|April 22, 1988
概括
1型星球细胞通过使用血小板衍生生长因子 (PDGF) 刺激2型星球细胞前代细胞的 oligodendrocyte 的增殖. 这种来自天体细胞的PDGF对于控制发育中的中枢神经系统中的髓化是必不可少的.
科学领域:
- 神经科学是一个神经科学.
- 发育生物学 发展生物学
- 细胞生物学 细胞生物学
背景情况:
- 双潜在的原始细胞 (O-2A原始细胞) 在成长中的老鼠视神经中产生寡细胞和2型天体细胞.
- 已知1型天体细胞在培养中诱导这些O-2A原始细胞的增殖.
研究的目的:
- 为了识别由1型天体细胞分泌的特定基因,刺激O-2A原始细胞的增殖.
- 阐明这个天体细胞衍生因子在髓化控制中的作用.
主要方法:
- 使用尺寸排除染色学对天体细胞条件介质的生物化学分析.
- 受体结合测试以评估与已知生长因子的竞争.
- 免疫沉以检测特定的蛋白质.
- 西方涂抹和RT-PCR检测PDGF及其mRNA在大脑组织中.
主要成果:
- 这种由天体细胞衍生的基因被确定为血小板衍生生长因子 (PDGF).
- 星细胞受条件介质的线粒体活性与PDGF相结合,与PDGF受体竞争,并被抗PDGF抗体中和.
- PDGF二元从介质中免疫降落,PDGFmRNA在质生成过程中被检测到在成长中的老鼠大脑中.
结论:
- 来自天体细胞的PDGF是O-2A原生细胞的关键线粒原体.
- 这一发现表明PDGF在调节中枢神经系统中寡腺细胞发育和髓化方面起着至关重要的作用.
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